tailieunhanh - báo cáo khoa học: " Differential gene expression in incompatible interaction between wheat and stripe rust fungus revealed by cDNA-AFLP and comparison to compatible interaction"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Differential gene expression in incompatible interaction between wheat and stripe rust fungus revealed by cDNA-AFLP and comparison to compatible interaction | Wang et al. BMC Plant Biology 2010 10 9 http 1471-2229 10 9 BMC Plant Biology RESEARCH ARTICLE Open Access Differential gene expression in incompatible interaction between wheat and stripe rust fungus revealed by cDNA-AFLP and comparison to compatible interaction . tA 1 1 w - 2 1 1 1 1 Xiaojie Wang Wei Liu Xianming Chen Chunlei Tang Yanling Dong Jinbiao Ma Xueling Huang Guorong Wei1 Qingmei Han1 Lili Huang1 Zhensheng Kang1 Abstract Background Stripe rust of wheat caused by Puccinia striiformis f. sp. tritici Pst is one of the most important diseases of wheat worldwide. Due to special features of hexaploid wheat with large and complex genome and difficulties for transformation and of Pst without sexual reproduction and hard to culture on media the use of most genetic and molecular techniques in studying genes involved in the wheat-Pst interactions has been largely limited. The objective of this study was to identify transcriptionally regulated genes during an incompatible interaction between wheat and Pst using cDNA-AFLP technique Results A total of 52 992 transcript derived fragments TDFs were generated with 64 primer pairs and 2 437 of them displayed altered expression patterns after inoculation with 1 787 up-regulated and 650 down-regulated. We obtained reliable sequences 100 bp for 255 selected TDFs of which 113 had putative functions identified. A large group of these genes shared high homology with genes involved in metabolism and photosynthesis to genes with functions related to disease defense and signal transduction and those in the remaining groups to genes involved in transcription transport processes protein metabolism and cell structure respectively. Through comparing TDFs identified in the present study for incompatible interaction and those identified in the previous study for compatible interactions 161 TDFs were shared by both interactions 94 were expressed specifically in the incompatible interaction of