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Báo cáo khoa học: Molecular identification and expression study of differentially regulated genes in the Pacific oyster Crassostrea gigas in response to pesticide exposure

The effects of pesticide contamination on the metabolism of marine mol-luscs are poorly documented. We investigated the response of a marine bivalve, the Pacific oyster,Crassostrea gigas, using a suppression subtrac-tive hybridization method to identify up- and down-regulated genes after a 30-day exposure period to herbicides (a cocktail of atrazine, diuron and isoproturon, and to the single herbicide glyphosate). A total of 137 unique differentially expressed gene sequences was identified, as well as their asso-ciated physiological process | iFEBS Journal Molecular identification and expression study of differentially regulated genes in the Pacific oyster Crassostrea gigas in response to pesticide exposure Arnaud Tanguy1 Isabelle Boutet1 2 Jean Laroche1 and Dario Moraga1 1 Laboratoire des Sciences de 1 Environnement Marin LEMAR UMR-CNRS 6539 Institut Universitaire Europeen de la Mer Universite de Bretagne Occidentale Plouzane France 2 UMR CNRS-IFREMER 5171 Genome Populations Interactions Adaptation Station Mediterraneenne de 1 Environnement Littoral Sete France Keywords Crassostrea gigas environment gene expression pesticides subtractive libraries Correspondence D. Moraga UMR-CNRS 6539 Laboratoire LEMAR Institut Universitaire Europeen de la Mer Universite de Bretagne Occidentale Place Nicolas Copernic F-29280 Plouzane France Fax 33 2 98 49 86 45 Tel 33 2 98 49 86 42 E-mail Dario.Moraga@univ-brest.fr Received 3 August 2004 revised 5 November 2004 accepted 12 November 2004 doi 10.1111 j.1742-4658.2004.04479.x The effects of pesticide contamination on the metabolism of marine molluscs are poorly documented. We investigated the response of a marine bivalve the Pacific oyster Crassostrea gigas using a suppression subtractive hybridization method to identify up- and down-regulated genes after a 30-day exposure period to herbicides a cocktail of atrazine diuron and isoproturon and to the single herbicide glyphosate . A total of 137 unique differentially expressed gene sequences was identified as well as their associated physiological process. The expression of 18 of these genes was analyzed by RT-PCR under laboratory experimental conditions. The metabolic functions they are associated with include xenobiotic detoxification energy production immune system response and transcription. This study provides a preliminary basis for studying the response of marine bivalves to long-term herbicide exposure in terms of regulated gene expression and characterizes new potential genetic markers of herbicide contamination.