tailieunhanh - báo cáo khoa học: "In vivo Molecular targeting effects of anti-Sp17ICG-Der-02 on hepatocellular carcinoma evaluated by an optical imaging system"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: In vivo Molecular targeting effects of anti-Sp17ICG-Der-02 on hepatocellular carcinoma evaluated by an optical imaging system | Li et al. Journal of Experimental Clinical Cancer Research 2011 30 25 http content 30 1 25 Journal of Experimental Clinical Cancer Research RESEARCH Open Access In vivo Molecular targeting effects of anti-Sp17-ICG-Der-02 on hepatocellular carcinoma evaluated by an optical imaging system Fang-qiu Li1 Shi-xin Zhang1 Lian-xiao An2 Yue-qing Gu2 Abstract Background As the expression of human sperm protein 17 Sp17 in normal tissue is limited and the function is obscure its aberrant expression in malignant tumors makes it to be a candidated molecular marker for tumor imaging diagnosis and targeting therapy of the aim of this research is to evaluate the targeting effects of anti-sperm protein 17 monoclonal antibody anti-Sp17 on cancer in vivo and investigate its usefulness as a reagent for molecular imaging diagnosis. Methods Immunohistochemistry was used to identify the expression of Sp17 in a hepatocellular carcinoma cell line and tumor xenograft specimens. A near infrared fluorescence dye ICG-Der-02 was covalently linked to anti-Sp17 for in vivo imaging. The immuno-activity of the anti-Sp17-ICG-Der-02 complex was tested in vitro by ELISA it was then injected into tumor-bearing nude mice through the caudal vein to evaluate its tumor targeting effect by near infrared imaging system. Results Overexpression of Sp17 on the surface of the hepatocellular carcinoma cell line SMMC-7721 was demonstrated. Anti-Sp17-ICG-Der-02 with immuno-activity was successfully synthesized. The immuno-activity and photo stability of anti-Sp17- ICG-Der-02 showed good targeting capability for Sp17 expressing tumor models SMMC-7721 in vivo and its accumulation in the tumor lasted for at least 7 days. Conclusions Anti-Sp17 antibody targeted and accumulated in Sp17 positive tumors in vivo which demonstrated its capability of serving as a diagnostic reagent. Introduction Cancer remains one of the leading causes of death in the world. Despite advances in our understanding of