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Báo cáo khoa học: " Detection and identification by PCR of Clostridium chauvoei in clinical isolates, bovine faeces and substrates from biogas plant"

Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú y đề tài: Detection and identification by PCR of Clostridium chauvoei in clinical isolates, bovine faeces and substrates from biogas plant. | Acta Veterinaria Scandinavica BioMed Central Research Detection and identification by PCR of Clostridium chauvoei in clinical isolates bovine faeces and substrates from biogas plant E Bagge 1 2 S Sternberg Lewerin3 and K-E Johansson1 2 Address Department of Bacteriology National Veterinary Institute SE-751 89 Uppsala Sweden 2Department of Biomedical Sciences and Veterinary Public Health Swedish University of Agricultural Sciences SE-751 89 Uppsala Sweden and 3Department of Disease Control Section for Epizootiology National Veterinary Institute SE-751 89 Uppsala Sweden Email E Bagge - elisabeth.bagge@sva.se S Sternberg Lewerin - Susanna.Lewerin@sva.se K-E Johansson - Karl-Erik.Johansson@bvf.slu.se Corresponding author Open Access Published 3 March 2009 Received 13 January 2009 Acta Veterinaria Scandinavica 2009 51 8 doi l0.ll86 l75l-0l47-5l-8 Accepted 3 March 2009 This article is available from http www.actavetscand.cOm content 5l l 8 2009 Bagge et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Clostridium chauvoei causes blackleg an acute disease associated with high mortality in ruminants. The apparent primary port of entry is oral during grazing on pasture contaminated by spores. Cases of blackleg can occur year after year on contaminated pastures. A method to determine the prevalence of C. chauvoei spores on pasture would be useful. The standard method for C. chauvoei detection is culture and biochemical identification which requires a pure culture. In most muscle samples from cattle dead from blackleg the amount of C. chauvoei in samples is high and the bacterium can easily be cultured although some samples may be contaminated. Detection by PCR would be faster and independent of contaminating .