tailieunhanh - Báo cáo khoa học: "Verification of the Combimatrix influenza detection assay for the detection of influenza A subtype during the 2007–2008 influenza season in Toronto, Canada"

Verification of the Combimatrix influenza detection assay for the detection of influenza A subtype during the 2007–2008 influenza season in Toronto, Canada | Virology Journal BioMed Central Open Access Short report Verification of the Combimatrix influenza detection assay for the detection of influenza A subtype during the 2007-2008 influenza season in Toronto Canada Shelly Bolotin 1 Ernesto Lombos1 Rani Yeung1 AliReza Eshaghi1 Joanne Blair1 and Steven J Drews1 2 3 Address 1Ontario Agency for Health Protection and Promotion 81 Resources Road Toronto Ontario M9P 3T1 Canada 2Department of Microbiology Mount Sinai Hospital 600 University Avenue Toronto Ontario M5G 1X5 Canada and 3Department of Laboratory Medicine and Pathobiology University of Toronto 100 College Street Room 110 Toronto Ontario M5G 1L5 Canada Email Shelly Bolotin - Ernesto Lombos - Rani Yeung - AliReza Eshaghi - Joanne Blair - Steven J Drews - Corresponding author Published 25 March 2009 Received 9 February 2009 Accepted 25 March 2009 Virology Journal 2009 6 37 doi l 743-422X-6-37 This article is available from http content 6 1 37 2009 Bolotin et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The increase in adamantine resistance in influenza A H3N2 and the emergence of oseltamivir resistance in influenza A H 1N1 has necessitated the use of rapid methodologies to detect influenza subtype. The purpose of this study was to evaluate the CombiMatrix influenza detection system compared to the FDA approved Luminex Respiratory virus panel RVP assay for influenza A subtyping. Verification of the CombiMatrix influenza detection system was carried out using the Luminex RVP assay as a reference method. A limit of detection LOD series was performed .