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Báo cáo y học: "Constitutive expression of cathepsin K in the human intervertebral disc: new insight into disc extracellular matrix remodeling via cathepsin K and receptor activator of nuclear factor-B ligand"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Constitutive expression of cathepsin K in the human intervertebral disc: new insight into disc extracellular matrix remodeling via cathepsin K and receptor activator of nuclear factor-B ligand. | Gruber et al. Arthritis Research Therapy 2011 13 R140 http arthritis-research.eom content 13 4 R140 RESEARCH ARTICLE Open Access Constitutive expression of cathepsin K in the human intervertebral disc new insight into disc extracellular matrix remodeling via cathepsin K and receptor activator of nuclear factor- B ligand 1 1 1 1 2 Helen E Gruber Jane A Ingram Gretchen L Hoelscher Natalia Zinchenko H James Norton and Edward N Hanley Jr1 Abstract Introduction Cathepsin K is a recently discovered cysteine protease which cleaves the triple helical domains of type I to II collagen. It has been shown to be up-regulated in synovial tissue from osteoarthritic and rheumatoid patients and is a component in normal and nonarthritic cartilage where it increases with aging. Studies on heart valve development have recently shown that receptor activator of nuclear factor-KB ligand RANKL acts during valve remodeling to promote cathepsin K expression. Since extracellular matrix remodeling is a critical component of disc structure and biomechanical function we hypothesized that cathepsin K and RANKL may be present in the human intervertebral disc. Methods Studies were performed following approval of the authors Human Subjects Institutional Review Board. Six annulus specimens from healthier Thompson grade I to II discs and 12 specimens from more degenerate grade III to IV discs were utilized in microarray analysis of RANKL and cathepsin K gene expression. Immunohistochemistry was also performed on 15 additional disc specimens to assess the presence of RANKL and cathepsin K. Results Cathepsin K gene expression was significantly greater in more degenerated grade III to IV discs compared to healthier grade I to II discs P 0.001 . RANKL was also identified with immunohistochemistry and molecular analyses. RANKL gene expression was also significantly greater in more degenerated discs compared to healthier ones P 0.0001 . A significant linear positive correlation was identified between .