tailieunhanh - Báo cáo y học: "Constitutive upregulation of the transforming growth factor-β pathway in rheumatoid arthritis synovial fibroblasts"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Constitutive upregulation of the transforming growth factor-β pathway in rheumatoid arthritis synovial fibroblasts. | Available online http content 9 3 R59 Research article Constitutive upregulation of the transforming growth factor-p pathway in rheumatoid arthritis synovial fibroblasts Dirk Pohlers1 Andreas Beyer2 3 Dirk Koczan4 Thomas Wilhelm2 5 Hans-Jurgen Thiesen4 and Raimund W Kinne1 Experimental Rheumatology Unit Department of Orthopedics Friedrich Schiller University Jena Eisenberg Germany 2Leibniz Institute for Age Research Fritz Lipmann Institute BeutenbergstraBe 11 Jena D-07745 Germany 3BIOTEC Technical University of Dresden Dresden 01602 Germany 4Institute of Immunology University of Rostock Schillingallee 69 Rostock D-18055 Germany institute of Food Research Colney Lane Colney Norwich NR4 7UA UK Corresponding author Dirk Pohlers Received 19 Dec 2006 Revisions requested 23 Jan 2007 Revisions received 22 May 2007 Accepted 26 Jun 2007 Published 26 Jun 2007 Arthritis Research Therapy 2007 9 R59 doi ar2217 This article is online at http content 9 3 R59 2007 Pohlers et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Genome-wide gene expression was comparatively investigated in early-passage rheumatoid arthritis RA and osteoarthritis OA synovial fibroblasts SFBs n 6 each using oligonucleotide microarrays mRNA protein data were validated by quantitative PCR qPCR and western blotting and immunohistochemistry respectively. Gene set enrichment analysis GSEA of the microarray data suggested constitutive upregulation of components of the transforming growth factor TGF -p pathway in RA SFBs with 2 hits in the top 30 regulated pathways. The growth factor TGF-p1 its receptor TGFBR1 the TGF-p binding proteins LTBP1 2 the TGF-p-releasing .