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Evaluation of an immunochromatographic test for the diagnosis of pulmonary tuberculosis in Madagascar
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Evaluation of an immunochromatographic test for the diagnosis of pulmonary tuberculosis in Madagascar
Anh Việt
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To improve the diagnosis of tuberculosis (TB), more rapid diagnostic techniques such as antibody detection based on immunochromatographic methods were developed. The objective of this study was to evaluate the performance of the SD Rapid TB kit for the diagnosis of active TB with serums from patients and close contact controls in Antananarivo, Madagascar. Findings: We conducted a population-based case-control study. The sera of 60 confirmed TB patients and 60 healthy contacts paired for sex and age were tested. The controls were healthy contacts who were exposed to TB but had no clinical or radiological evidence of TB. The SD Rapid TB kit was used with. | Said Toihir et al. BMC Research Notes 2011 4 403 http www.biomedcentral.eom 1756-0500 4 403 BMC Research Notes SHORT REPORT Open Access Evaluation of an immunochromatographic test for the diagnosis of pulmonary tuberculosis in Madagascar Al-habib O Said Toihir 1 Vincent Richard2 Vaomalala Raharimanga2 Samuel H Andrianarisoa3 Gabriel M Ranjalahy4 Herimanana Ramarokoto1 and Voahangy Rasolofo1 Abstract Background To improve the diagnosis of tuberculosis TB more rapid diagnostic techniques such as antibody detection based on immunochromatographic methods were developed. The objective of this study was to evaluate the performance of the SD Rapid TB kit for the diagnosis of active TB with serums from patients and close contact controls in Antananarivo Madagascar. Findings We conducted a population-based case-control study. The sera of 60 confirmed TB patients and 60 healthy contacts paired for sex and age were tested. The controls were healthy contacts who were exposed to TB but had no clinical or radiological evidence of TB. The SD Rapid TB kit was used with serum samples according to the instructions of the manufacturer. Sensitivity specificity accuracy and predictive values were calculated using culture on Lovenstein-Jensen media as gold standard . In this study the sensitivity of the test was 55 and the specificity 90 . The positive predictive value and the negative predictive value were 84.61 and 66.66 respectively for diagnosis of pulmonary TB. Conclusions This study shows that the SD Rapid TB test is a simple and fast method. This test has a good specificity and could therefore help rule in TB if positive but lacks adequate sensitivity. Background Tuberculosis TB poses a significant health threat worldwide with an estimated 9 million new cases and 2 million deaths every year worldwide 1 . Morbidity associated with TB has been decreasing but Human Immunodeficiency Virus HIV associated TB and multidrug resistant MDR are on the rise 2 . According to the 2010 World .
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