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Báo cáo y học: "Use of HLA-B27 tetramers to identify low-frequency antigen-specific T cells in Chlamydia-triggered reactive arthritis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Use of HLA-B27 tetramers to identify low-frequency antigen-specific T cells in Chlamydia-triggered reactive arthritis. | Available online http arthritis-research.eom content 6 6 R521 Research article Use of HLA-B27 tetramers to identify low-frequency antigen-specific T cells in Chlamydia-triggered reactive arthritis Heiner Appel1 Wolfgang Kuon1 Maren Kuhne1 Peihua Wu1 2 Stefanie Kuhlmann1 Simon Kollnberger3 Andreas Thiel2 Paul Bowness3 and Joachim Sieper1 2 1Charite Berlin Campus Benjamin Franklin Department for Gastroenterology Infectiology and Rheumatology Berlin Germany 2Deutsches Rheumaforschungszentrum Berlin Germany 3MRC HIU Institute of Molecular Medicine John Radcliffe Hospital Oxford UK Corresponding author Heiner Appel heinerappel@yahoo.com Received 7 Apr 2004 Revisions requested 13 May 2004 Revisions received 8 Jun 2004 Accepted 2 Jul 2004 Published 23 Sep 2004 Arthritis Res Ther 2004 6 R521-R534 DOI 10.1186 ar1221 2004 Appel et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is cited. Open Access Abstract Reports of the use of HLA-B27 peptide tetrameric complexes to study peptide-specific CD8 T cells in HLA-B27 -related diseases are rare. To establish HLA-B27 tetramers we first compared the function of HLA-B27 tetramers with HLA-A2 tetramers by using viral epitopes. HLA-B27 and HLA-A2 tetramers loaded with immunodominant peptides from Epstein-Barr virus were generated with comparable yields and both molecules detected antigen-specific CD8 T cells. The application of HLA-B27 tetramers in HLA-B27-related diseases was performed with nine recently described Chlamydia-derived peptides in synovial fluid and peripheral blood to examine the CD8 T cell response against Chlamydia trachomatis antigens in nine patients with Chlamydia-triggered reactive arthritis Ct-ReA . Four of six HLA-B27 Ct-ReA patients had specific synovial T cell binding to at least .