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Báo cáo y học: "Comparative study of the persistence of anti-HIV activity of deoxynucleoside HIV reverse transcriptase inhibitors after removal from culture"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Comparative study of the persistence of anti-HIV activity of deoxynucleoside HIV reverse transcriptase inhibitors after removal from culture | AIDS Research and Therapy BioMed Central Research Comparative study of the persistence of anti-HIV activity of deoxynucleoside HIV reverse transcriptase inhibitors after removal from culture Elijah Paintsil1 2 Susan P Grill2 Ginger E Dutschman2 and Yung- Chi Cheng 2 Address Department of Pediatrics Yale University School of Medicine New Haven CT 06520 USA and 2Department of Pharmacology Yale University School of Medicine New Haven CT 06520 USA Email Elijah Paintsil - elijah.paintsil@yale.edu Susan P Grill - susan.grill@yale.edu Ginger E Dutschman - ginger.dutschman@yale.du Yung-Chi Cheng - yccheng@yale.edu Corresponding author Open Access Published 22 April 2009 Received 8 January 2009 AIDS Research and Therapy 2009 6 5 doi 10.1186 1742-6405-6-5 Accepted 22 April 2009 This article is available from http www.aidsrestherapy.cOm content 6 1 5 2009 Paintsil et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Most in vitro assays of drug potency may not adequately predict the performance in vivo. Methods to assess the persistence of antiviral activity of deoxynucleoside analogs which require intracellular activation to the active metabolites that can persist in cells will be important for designing dosages combination regimens and assessing treatment compliance. Using an HIV-IIIB TZM-bl indicator cell culture system we assessed the ability of an inhibitor to protect cells from infection and to delay viral rebound after removal of inhibitor from culture. Results The order of protection of cells from HIV-infection was 4 -Ed4T LFD4C DDI D4T 3TC AZT FTC NVP. The fold-increase in EC50 to delay viral rebound was DDI 4 -Ed4T LFD4C FTC D4T 3TC NVP AZT. The ranking of persistence of anti-HIV activity of the .