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Báo cáo khoa học: "Diagnosis of Helicobacter spp. infection in canine stomach"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Diagnosis of Helicobacter spp. infection in canine stomach | J. Vet. Set. 2007 8 2 139-145 JOURNAL OF Veterinary Science Diagnosis of Helicobacter spp. infection in canine stomach w. Prachasilpchai1 s. Nuanualsuwan2 T. Chatsuwan3 s. Techangamsuwans. Wangnaitham1 A. Sailasuta1 1 Department of Pathology and2Department of Veterinary Public Health Faculty of Veterinary Science and 3Department of Microbiology Faculty of Medicine Chulalongkorn University Bangkok 10330 Thailand A total of 75 biopsied samples of cardia fundus body and pyloric antrum from necropsied dogs that were submitted to the Department of Pathology Faculty of Veterinary Science Chulalongkorn University from April 2003 to June 2004 were investigated. The objectives of this study were to determine the prevalence of Helicobacter spp. in canine stomach by polymerase chain reaction PCR in comparison to histochemistry versus immunohistochemistry IHC and to correlate these diagnostic methods with the clinical significance in infected dogs. Histopathological results revealed 60.0 45 75 of samples to be positive and consisted of mild gastritis in 64.44 29 45 moderate gastritis in 11.11 5 45 and severe gastritis in 24.44 11 45 . The proportion showing no histopathological lesions was 40.0 30 75 . Helicobacter spp. were localized to the luminal crypt in 18.67 14 75 gastric pit in 22.67 17 75 gastric gland in 21.33 16 75 and gastric epithelium in 8 6 75 . The percentages of positive samples of Helicobacter spp. diagnosed by hematoxylin and eosin stain H E Warthin Starry stain WSS IHC with rabbit polyclonal anti- . pylori antibody and PCR were 17.3 13 75 46.7 35 75 30.7 23 75 and 10.7 8 75 respectively. No significant differences weree observed in histopathological changes in portions of the stomach p 0.05 . The diagnosis of Helicobacter spp. by PCR in comparison to that by wss and me was not significantly different Ip 0.05 . There were no relationships between pathological studies using H E wss and IHC and especially between PCR and clinical signs of Helicobacter spp. .