Đang chuẩn bị liên kết để tải về tài liệu:
Báo cáo khoa học: "Mutations of p53 Tumor Suppressor Gene in Spontaneous Canine Mammary Tumors"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Mutations of p53 Tumor Suppressor Gene in Spontaneous Canine Mammary Tumors | J. Vet. Sci. 2002 3 4 321-325 JOURNAL OF Veterinary Science Mutations of p53 Tumor Suppressor Gene in Spontaneous Canine Mammary Tumors Chung-Ho Lee and Oh-Kyeong Kweon Department of Veterinary Surgery College of Veterinary Medicine Seoul National University San 56-1 Shillim 9-dong Kwanak-gu Seoul 151-742 Korea Received July 13 2002 Accepted November 27 2002 Abstract Mutation of the p53 tumor suppressor gene has been related in the pathogenesis of numerous human and canine cancers including breast cancers and mammary tumors. We have investigated exons 5-8 of the p53 gene for mutations in 20 spontaneous canine mammary tum ors using polym erase ch ain reaction P CR with direct sequence an alysis to evaluate the role of this gene in canine mammary tumorigenesis and analyzed to compare with other clinicopathological parameters including age histology stage recurrence and death from tum or. Four missense one case had two missense mutations and one nonsense mutations were detected in 10 malignant lesions 40 and two m iss en se an d on e silen t m u ta tion s w e re foun d in 10 benign mammary tumors 30 . Five of the missense mutations were located in highly conserved dom ains II III IV and V. After a follow-up period four dogs show ed a progression and three of these p atients revealed death from m amm ary carcinom a with p53 m u t a ti o n . Th e s e re sults d em on stra te d th a t th e p53 gene mutations might be involved in the development of canine mammary tum ors and contribute to the prognostic status in canine mammary carcinom as. Key words dog mammary tumor p53 gene mutation Introduction Recent advances in tumor biology have identified a number of markers that may form a basis for tumor stratification 7 10 25 . E specia lly numerous st u dies have been focu sed on the investigation of the significant role of the p53 tumor suppressor gene in the tumorigenesis of human and canine cancer s. The p53 gene mutates most Corresponding author Oh-Kyeong Kweon Department