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Báo cáo sinh học: "Curcumin reduces expression of Bcl-2, leading to apoptosis in daunorubicin-insensitive CD34+ acute myeloid leukemia cell lines and primary sorted CD34+ acute myeloid leukemia cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Curcumin reduces expression of Bcl-2, leading to apoptosis in daunorubicin-insensitive CD34+ acute myeloid leukemia cell lines and primary sorted CD34+ acute myeloid leukemia cells | Rao et al. Journal of Translational Medicine 2011 9 71 http www.translational-medicine.eom content 9 1 71 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Curcumin reduces expression of Bcl-2 leading to apoptosis in daunorubicin-insensitive CD34 acute myeloid leukemia cell lines and primary sorted CD34 acute myeloid leukemia cells 12 2 3 4 12 5 12 12 Jia Rao Duo-Rong Xu Fei-Meng Zheng Zi-Jie Long Sheng-Shan Huang Xing Wu Wei-Hua Zhou Ren-Wei Huang1 2 and Quentin Liu1 2 4 Abstract Background Acute myeloid leukemia AML is an immunophenotypically heterogenous malignant disease in which CD34 positivity is associated with poor prognosis. CD34 AML cells are 10-15-fold more resistant to daunorubicin DNR than CD34- AML cells. Curcumin is a major component of turmeric that has shown cytotoxic activity in multiple cancers however its anti-cancer activity has not been well studied in DNR-insensitive CD34 AML cells. The aim of this study was to therefore to explore curcumin-induced cytotoxicity in DNR-insensitive CD34 AML cell lines KG1a Kasumi-1 DNR-sensitive U937 AML cells and primary CD34 AML bone-marrow-derived cells. Methods Primary human CD34 cells were isolated from peripheral blood mononuclear cells or bone marrow mononuclear cells using a CD34 MicroBead kit. The growth inhibitory effects of curcumin were evaluated by MTT and colony-formation assays. Cell cycle distribution was examined by propidium iodide PI assay. Apoptosis was analyzed by Wright-Giemsa Hoechst 33342 and Annexin-V PI staining assays. The change in mitochondrial membrane potential MMP was examined by JC-1 staining and flow cytometry. Expression of apoptosis-related proteins was determined by reverse transcription-polymerase chain reaction and Western blotting. Short interfering RNA siRNA against Bcl-2 was used in CD34 KG1a and Kasumi-1 cells incubated with without DNR. Results Curcumin inhibited proliferation and induced apoptosis and G1 S arrest in both DNR-insensitive KG1a Kasumi-1 and .