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Báo cáo y học: " Identification of novel transcripts with differential dorso-ventral expression in Xenopus gastrula using serial analysis of gene expression"

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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Identification of novel transcripts with differential dorso-ventral expression in Xenopus gastrula using serial analysis of gene expression. | Open Access Identification of novel transcripts with differential dorso-ventral expression in Xenopus gastrula using serial analysis of gene expression Fernando Faunes Natalia Sanchez Javier Castellanos Ismael A Vergara Francisco Melo and Juan Larraín Addresses Center for Cell Regulation and Pathology and Center for Aging and Regeneration Facultad de Ciencias Biológicas Pontificia Universidad Católica de Chile Alameda 340 Santiago 8331150 Chile. tLaboratorio de Bioinformatica Molecular Depto. Genética Molecular y Microbiología Facultad de Ciencias Biológicas Pontificia Universidad Católica de Chile Alameda 340 Santiago 8331150 Chile. Correspondence Juan Larraín. Email jlarrain@bio.puc.cl Published II February 2009 Genome Biology 2009 10 RI5 doi I0.II86 gb-2009- I0-2-rI5 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2009 I0 2 RI5 Received 3 October 2008 Revised 25 November 2008 Accepted II February 2009 2009 Faunes et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Recent evidence from global studies of gene expression indicates that transcriptomes are more complex than expected. Xenopus has been typically used as a model organism to study early embryonic development particularly dorso-ventral patterning. In order to identify novel transcripts involved in dorso-ventral patterning we compared dorsal and ventral transcriptomes of Xenopus tropicalis at the gastrula stage using serial analysis of gene expression SAGE . Results Of the experimental tags 54.5 were confidently mapped to transcripts and I25 showed a significant difference in their frequency of occurrence between dorsal and ventral libraries. We selected 20 differentially expressed

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