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báo cáo khoa học: " Mutations in a plastid-localized elongation factor G alter early stages of plastid development in Arabidopsis thaliana"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Mutations in a plastid-localized elongation factor G alter early stages of plastid development in Arabidopsis thaliana | BMC Plant Biology BioMed Central Research article Mutations in a plastid-localized elongation factor G alter early stages of plastid development in Arabidopsis thaliana Nicholas J Ruppel and Roger P Hangarter Open Access Address Department of Biology Indiana University Bloomington IN 47405 USA Email Nicholas J Ruppel - nruppel@indiana.edu Roger P Hangarter - rhangart@indiana.edu Corresponding author Published 13 July 2007 Received 1 December 2006 BMC Plant Biology 2007 7 37 doi 10.1186 1471-2229-7-37 Accepted 13 July 2007 This article is available from http www.biomedcentral.com 1471-2229 7 37 2007 Ruppel and Hangarter licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.Org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Proper development of plastids in embryo and seedling tissues is critical for plant development. During germination plastids develop to perform many critical functions that are necessary to establish the seedling for further growth. A growing body of work has demonstrated that components of the plastid transcription and translation machinery must be present and functional to establish the organelle upon germination. Results We have identified Arabidopsis thaliana mutants in a gene that encodes a plastid-targeted elongation factor G SCO1 that is essential for plastid development during embryogenesis since two T-DNA insertion mutations in the coding sequence sco1-2 and sco1-3 result in an embryo-lethal phenotype. In addition a point mutation allele sco1-1 and an allele with a T-DNA insertion in the promoter sco1-4 of SCO1 display conditional seedling-lethal phenotypes. Seedlings of these alleles exhibit cotyledon and hypocotyl albinism due to improper chloroplast development and normally die shortly after germination. However when germinated on