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Báo cáo khoa học: " Development of a novel monoclonal antibody with reactivity to a wide range of Venezuelan equine encephalitis virus strains"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Development of a novel monoclonal antibody with reactivity to a wide range of Venezuelan equine encephalitis virus strains | Virology Journal BioMed Central Research Development of a novel monoclonal antibody with reactivity to a wide range of Venezuelan equine encephalitis virus strains Lyn M O Brien Cindy D Underwood-Fowler Sarah A Goodchild Amanda L Phelps and Robert J Phillpotts Open Access Address Biomedical Sciences Department Defence Science and Technology Laboratory Porton Down Salisbury Wiltshire SP4 0JQ UK Email Lyn M O Brien - lmobrien@dstl.gov.uk Cindy D Underwood-Fowler - cdufowler@dstl.gov.uk Sarah A Goodchild - sagoodchild@dstl.gov.uk Amanda L Phelps - alphelps@dstl.gov.uk Robert J Phillpotts - bjphillpotts@dstl.gov.uk Corresponding author Published 19 November 2009 Received 19 June 2009 Accepted 19 November 2009 Virology Journal 2009 6 206 doi l0.ll86 I743-422X-6-206 This article is available from http www.virologyj.com content 6 1 206 2009 O Brien et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background There is currently a requirement for antiviral therapies capable of protecting against infection with Venezuelan equine encephalitis virus VEEV as a licensed vaccine is not available for general human use. Monoclonal antibodies are increasingly being developed as therapeutics and are potential treatments for VEEV as they have been shown to be protective in the mouse model of disease. However to be truly effective the antibody should recognise multiple strains of VEEV and broadly reactive monoclonal antibodies are rarely and only coincidentally isolated using classical hybridoma technology. Results In this work methods were developed to reliably derive broadly reactive murine antibodies. A phage library was created that expressed .