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báo cáo khoa học: " Characterization and structural analysis of wild type and a non-abscission mutant at the development funiculus (Def) locus in Pisum sativum L"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Characterization and structural analysis of wild type and a non-abscission mutant at the development funiculus (Def) locus in Pisum sativum L | BMC Plant Biology BioMed Central Research article Characterization and structural analysis of wild type and a non-abscission mutant at the development funiculus Def locus in Pisum sativum L Kwadwo Owusu Ayeh 1 YeonKyeong Leef1 Mike J Ambrose2 and Anne Kathrine Hvoslef-Eide 1 Address Department of Plant and Environmental Sciences Norwegian University of Life Sciences PO BOX 5003 1432 Aas Norway and 2Department of Crops Genetics John Innes Centre Norwich Research Park Colney Lane NR4 7UH Norwich UK Email Kwadwo Owusu Ayeh - kwawdo.owusu.ayeh@umb.no YeonKyeong Lee - yeonkyeong.lee@umb.no Mike J Ambrose - mike.ambrose@bbsrc.ac.uk Anne Kathrine Hvoslef-Eide - trine.hvoslef-eide@umb.no Corresponding author fEqual contributors Open Access Published 23 June 2009 Received 25 August 2008 BMC Plant Biology 2009 9 76 doi 10.1186 1471-2229-9-76 Accepted 23 June 2009 This article is available from http www.biomedcentral.cOm 1471-2229 9 76 2009 Ayeh et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background In pea seeds Pisum sativum L. the Def locus defines an abscission event where the seed separates from the funicle through the intervening hilum region at maturity. A spontaneous mutation at this locus results in the seed failing to abscise from the funicle as occurs in wild type peas. In this work structural differences between wild type peas that developed a distinct abscission zone AZ between the funicle and the seed coat and non-abscission def mutant were characterized. Results A clear abscission event was observed in wild type pea seeds that were associated with a distinct double palisade layers at the junction between the seed coat and funicle. Generally mature seeds fully developed an AZ which was not present in .