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Isolation and screening of lactic acid bacteria for acidic pH and bile tolerance

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As many as 25 lactic acid bacterial strains were isolated from various sources. These isolates were investigated for their tolerance to low pH (3.0, 4.0 and 5.0) and different bile concentrations (0.3 %, 0.4 %, 0.75 % and 1.0 %). Among 25 isolates, strain 42, 45 and 48 showed very high tolerance to acidic pH and the same isolates also tolerated high level of bile salt concentration up to 1.0 per cent. Thus, high resistance to low pH values and to higher concentrations of bile salts enables these strains to survive in the stomach and intestinal environment and to colonize the gut of the host, possess a promising probiotic potential. Hence, these isolates hold potential to be used as probiotics. |

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