tailieunhanh - Isolation, screening and the influence of cultivation factors on cellulase of bacteria isolated from termites gut
The results from cultivation study revealed that M3 medium containing rice bran 1 %, soy flour 1 %, casein 1 % and 1 % NaCl was the best medium for cellulase production of B. subtilis G4. At optimal conditions for G4 which were inoculums ratio 1 %, cultivation temperature 37ºC, cultivation time 72 hours, shaking speed 150 rpm and initial pH medium 7, CMCase and FPase activity reached U/ml and U/ml, respectively. | Journal of Science and Technology 54 (4A) (2016) 89-96 ISOLATION, SCREENING AND THE INFLUENCE OF CULTIVATION FACTORS ON CELLULASE OF BACTERIA ISOLATED FROM TERMITES GUT Hien Thi Nguyen1, *, Xuan Thi Thanh Dao2, Lang Thi Nguyen1, Nga Thi To1, Ha Thanh Le1 1 Hanoi University of Science and Technology, No 1 Dai Co Viet, Bach Khoa Ward, Hai Ba Trung Dist, Hanoi 2 Vinh University, No182 Lê Duẩn, Vinh City, Nghe An Province * Email: nguyenhien15011990@ Received: 15 August 2016; Accepted for publication: 5 October 2016 ABSTRACT From five termites guts samples collected in different places in Ha Noi and Vinh, 11 isolates which formed halo on agar plates with CMC were isolated. Among them strain G4 possessed the highest CMCase activity. Based on morphology and 16S rRNA gene sequences analysis, G4 was identified as Bacillus subtilis G4. The results from cultivation study revealed that M3 medium containing rice bran 1 %, soy flour 1 %, casein 1 % and 1 % NaCl was the best medium for cellulase production of B. subtilis G4. At optimal conditions for G4 which were inoculums ratio 1 %, cultivation temperature 37ºC, cultivation time 72 hours, shaking speed 150 rpm and initial pH medium 7, CMCase and FPase activity reached U/ml and U/ml, respectively. Keywords: termites gut, bacteria, cellulase, CMCase, FPase. 1. INTRODUCTIONS Cellulose is the most abundant renewable biomass on earth (100 billion tons of dry volumes/year). Cellulose is degraded by cellulase by hydrolyzing β – 1,4 linkages in cellulose chains. Three types of cellulases based on the type of catalysed reaction are endocellulase (CMCase), exocellulase and β-glucosidase (cellobiase). Cellulase is the most applied enzyme in the food industry, paper industry and textile industry . to hydrolyze cellulose-rich materials for improving the extraction efficiency and product quality. However, the most important applications of cellulase is involving in the hydrolysis of raw materials such as straw, .
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