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Effect of astaxanthin supplementation on semen (Karan Fries Bulls) Storage at 5 °C

The study was conducted on three (n=3) adult healthy Karan Fries bulls (4-6 years) maintained at Artificial Breeding Research Centre (ABRC), Indian Council of Agricultural Research-National Dairy Research Institute (ICAR-NDRI), Karnal, Haryana. Six ejaculates from each bull were collected at weekly interval using artificial vagina (42-45 °C). | Int.J.Curr.Microbiol.App.Sci 2017 6 5 23-28 International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 6 Number 5 2017 pp. 23-28 Journal homepage http www.ijcmas.com Original Research Article https doi.org 10.20546 ijcmas.2017.605.003 Effect of Astaxanthin Supplementation on Semen Karan Fries Bulls Storage at 5 C Simson Soren Sohan Vir Singh and Sunil Kumar Animal Physiology Division Climate Resilient Livestock Research Centre Indian Council of Agricultural Research-National Dairy Research Institute ICAR-NDRI Karnal Haryana -132001 India Corresponding author ABSTRACT Astaxanthin AX is a xanthophyll carotenoid having the powerful antioxidant ability with much beneficial health effects. The present study was conducted to observe the effect of AX on sperm quality of Karan Fries Tharparkar X Holstein Friesian bulls during storage Keywords at 5 C. The neat semen was diluted with Tris egg yolk citric acid fructose TEYCAF Astaxanthin extender and equally divided into two parts i.e. control and AX supplementation. The Semen Storage percentage of progressive motility at 0 24 48 and 72 hours were 78.75 1.25 vs.78.75 Refrigerator 1.25 77.50 1.44 vs. 72.5 2.5 77.50 1.44 vs. 71.25 1.25 and 68.75 1.25 vs. 66.25 temperature. 2.39 in AX supplemented vs. control respectively. The percentage of live spermatozoa at 0 24 48 and 72 hours were as 88.69 0.09 vs. 88.12 1.32 85.93 0.59 vs. 83.76 Article Info 0.61 82.95 1.31 vs. 78.57 0.70 and 80.92 1.26 vs. 74.07 0.95 in AX supplemented vs. control respectively. The concentration of catalase CAT enzyme in supernatant was Accepted reduced p Int.J.Curr.Microbiol.App.Sci 2017 6 5 23-28 membrane integrity p 0.05 in rams diluted preliminary study was conducted to observe semen during the storage period of 72 hours the effect of AX a potent antioxidant at 5 C Fang et al. 2015 . Malondialdehyde supplementation on semen preservation of product of lipid peroxidation and reactive Karan Fries bulls at refrigerator .