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Isolation of bacteria from the vaginal aspirates of cyclic, acyclic, endometritic and pregnant crossbred cows

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A study was carried out on vaginal secretions/aspirates from infertile (acyclic and endometritic) crossbred cows from field and healthy cyclic as well as pregnant crossbred cows of University farm to identify the vaginal microorganisms based on routine cultural examination. The work was carried out on total 36 crossbred cows covering six each regular cyclic (proestrus, estrus, metestrus, diestrus), acyclic, endometritic and 3, 6 and 9 months pregnant animals. The samples of cervico-vaginal mucus/discharge during estrus/ endometritis, and vaginal washings during other phases of estrous cycle as well as pregnancy were collected aseptically using syringe and pipette method. The samples obtained were soon processed for cultural isolation on Blood agar and MacConkey agar, and identified using Gram’s staining and biochemical tests. | Isolation of bacteria from the vaginal aspirates of cyclic, acyclic, endometritic and pregnant crossbred cows

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