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A cross-sectional study on the occurrence of Coxiella Burnetii infection in a dairy farm, Bareilly, India
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Q fever is highly infectious bacterial zoonoses caused by Coxiella burnetii and remains largely neglected and underreported in various states of India. The present cross-sectional study employing a simple random sampling approach analysed a total of 324 samples (108 blood, 108 sera and 108 vaginal swabs) from cattle (n=108) employing of PCR and ELISA of cattle dairy farm from Bareilly, Uttar Pradesh, India. Besides, 18 environmental samples (animal feed-05, soil-04, drainage water-05 and drinking water-04) from the premises of the farm were also collected. On screening of cattle samples by trans-PCR and com1-PCR revealed positivity for C. burnetii DNA in 9.25% (10/108) and 5.55% (6/108) samples of cattle blood; 12.03% (13/108) and 5.55% (6/108) of sera, and 12.96% (14/108) and 06.48% (7/108) of vaginal swabs, respectively. Screening of cattle on the farm by commercial i-ELISA kit revealed antibodies against C. burnetii in the serum samples of 14.81% (16/108) cattle population. | A cross-sectional study on the occurrence of Coxiella Burnetii infection in a dairy farm, Bareilly, India