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Enhancing production of lignin peroxidase from white rot fungi employing statistical optimization and evaluation of its potential in delignification of crop residues

A high demand for fungal lignin peroxidases on account of their innumerable biotechnological applications necessitates enhanced production This paper describes the enhanced production of lignin peroxidase by three isolates (LPS1, LPS2 and LPS3) of white rot fungi for delignification of crop residues. Initial screening of medium components was performed using a Plackett–Burman design and the variables with statistically significant effects such as pH, temperature, carbon source, nitrogen source, inoculums size, incubation period, inhibitors on lignin peroxidase production were identified. The optimized temperature was 300C with pH 3 for LiP production in all three isolates (LPS1, LPS2 and LPS3). | Enhancing production of lignin peroxidase from white rot fungi employing statistical optimization and evaluation of its potential in delignification of crop residues