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Cytotoxic and apoptotic effects of ceranib-2 offering potential for a new antineoplastic agent in the treatment of cancer cells

The tumours typically form in the presence of connective tissue and are of key consideration when assessing research priorities for future healthcare management. Ceramidase inhibitors, such as ceranib-2, have demonstrated capacity to interfere with cellular DNA functionality, initiating apoptosis in many cancer cell lines. | Turkish Journal of Biology Turk J Biol (2014) 38: 916-921 © TÜBİTAK doi:10.3906/biy-1405-36 http://journals.tubitak.gov.tr/biology/ Research Article Cytotoxic and apoptotic effects of ceranib-2 offering potential for a new antineoplastic agent in the treatment of cancer cells 1, 1 2 3 3 Djanan VEJSELOVA *, Hatice Mehtap KUTLU , Gökhan KUŞ , Selda KABADERE , Ruhi UYAR 1 Department of Biology, Faculty of Science, Anadolu University, Eskişehir, Turkey 2 Department of Health, Faculty of Open Education, Anadolu University, Eskişehir, Turkey 3 Department of Physiology, Faculty of Medicine, Osmangazi University, Eskişehir, Turkey Received: 13.05.2014 Accepted: 21.07.2014 Published Online: 24.11.2014 Printed: 22.12.2014 Abstract: Fibrocarcinomas are malignant tumours that originate in mesenchymal cells. The tumours typically form in the presence of connective tissue and are of key consideration when assessing research priorities for future healthcare management. Ceramidase inhibitors, such as ceranib-2, have demonstrated capacity to interfere with cellular DNA functionality, initiating apoptosis in many cancer cell lines. The enzyme ceramidase can regulate cellular levels of sphingosine and sphingosine-1-phosphate by controlling hydrolysis of ceramide. The present study investigates the antigrowth effects of ceranib-2 on mouse embryonic fibroblast cells (NIH/3T3 normal cell line) and rat embryonic fibroblast cells (5RP7 cancer cell line). Research was conducted using colorimetric 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, confocal microscopy, and transmission electron microscopy. The results indicate that in vitro fibrocarcinomas have the potential to undergo apoptotic death when influenced by the antigrowth behaviour of ceranib-2. This is true even of cells exposed only to minimal doses of ceranib-2. Indicators of apoptotic death, specifically presentation of a pyknotic nucleus, were observed by confocal micrographs in .