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Regeneration and histological analysis of regeneration in bottle gourd (Lagenaria siceraria (Molina) Stand.)

Two different types of explant (proximal and flamingo-bill) from Emphasis seedlings, a hybrid cultivar of bottle gourd (Lagenaria siceraria (Molina) Stand.), germinated under dark and light conditions were cultured on 9 regeneration MS media containing various combinations of BA (0, 1.0, and 2.0 mg l–1) and IAA (0, 0.25, and 0.5 mg l–1). | Turk J Agric For 33 (2009) 165-172 © TÜBİTAK doi:10.3906/tar-0806-29 Regeneration and Histological Analysis of Regeneration in Bottle Gourd (Lagenaria siceraria (Molina) Stand.) Yeşim YALÇIN MENDİ1, Muzaffer İPEK2, Nihal BUZKAN3, Yıldız AKA KAÇAR1, Sebahattin ÇÜRÜK4 1 Laboratory of Biotechnology, Department of Horticulture, Faculty of Agriculture, University of Çukurova, Balcalı, Adana - TURKEY 2 Department of Horticulture, Faculty of Agriculture, University of Selçuk, Konya - TURKEY 3 Department of Plant Protection, Faculty of Agriculture, Kahramanmaraş Sütçü İmam University, 46060 Kahramanmaraş - TURKEY 4 Department of Horticulture, Faculty of Agriculture, Mustafa Kemal University, Antakya - TURKEY Received: 19.06.2008 Abstract: Two different types of explant (proximal and flamingo-bill) from Emphasis seedlings, a hybrid cultivar of bottle gourd (Lagenaria siceraria (Molina) Stand.), germinated under dark and light conditions were cultured on 9 regeneration MS media –1 –1 containing various combinations of BA (0, 1.0, and 2.0 mg l ) and IAA (0, 0.25, and 0.5 mg l ). Comparison of the explant types showed that the flamingo-bill type explant had better shoot formation than did the proximal explant. The MS medium containing –1 1 mg l of BA was optimal for shoot formation capacity when flamingo-bill type explants germinated in the dark (44%) and light (36%) were used. Histological analysis showed that explant cell division began after 3 days in regeneration medium and formation of primordium was observed in the tissues in culture between days 5 and 7. Differentiation of meristimatic structures was first observed after 9 days and development completed after 9-12 days in the culture. Key Words: Bottle Gourd, Lagenaria siceraria, regeneration, histology, explant type Su Kabağında (Lagenaria siceraria (Molina) Stand.) Rejenerasyon ve Rejenerasyonun Histolojik Analizi Özet: Bu çalışmada, hibrit su kabağı (Lagenaria siceraria (Molina) Stand.) çeşidi olan .