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Production of cellulase by Pleurotus ostreatus and Pleurotus sajor-caju in solid state fermentation of lignocellulosic biomass

Bioconversion of cellulosic components into fermentable sugars has currently been accomplished with the help of the microbial enzyme, cellulase. In this study, Pleurotus ostreatus (Jacquin ex Fr.) Kummer and Pleurotus sajor-caju (Fr.) Singer were tested for cellulase production by solid state fermentation (SSF) using saw dust, sugarcane bagasse, and paddy straw as substrate. | Md. I. KHALIL, M. M. HOQUE, M. A. BASUNIA, N. ALAM, Md. A. KHAN Research Article Turk J Agric For 35 (2011) 333-341 © TÜBİTAK doi:10.3906/tar-1002-684 Production of cellulase by Pleurotus ostreatus and Pleurotus sajor-caju in solid state fermentation of lignocellulosic biomass Md. Ibrahim KHALIL1,*, Mia Mahmudul HOQUE1, Mafroz Ahmed BASUNIA1, Nadia ALAM2, Md. Asaduzzaman KHAN2,3,* 1 Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka 1342 - BANGLADESH 2 National Mushroom Development and Extension Centre, Savar, Dhaka 1340 - BANGLADESH 3 Department of Biochemistry, School of Biological Science and Technology, Central South University, Changsha, Hunan 410013 - CHINA Received: 01.03.2010 Abstract: Bioconversion of cellulosic components into fermentable sugars has currently been accomplished with the help of the microbial enzyme, cellulase. In this study, Pleurotus ostreatus (Jacquin ex Fr.) Kummer and Pleurotus sajor-caju (Fr.) Singer were tested for cellulase production by solid state fermentation (SSF) using saw dust, sugarcane bagasse, and paddy straw as substrate. To enhance production, the substrates were pretreated with 1% and 4% NaOH solutions. Supernatants of extracted enzyme solutions were used to estimate reducing sugar and soluble protein, as well as enzyme activities. Maximum production of reducing sugar and soluble protein were recorded in 12 and 10 day fermented products, respectively. In the production of reducing sugars, P. ostreatus (42.8 mg g-1) was found to be more efficient than P. sajor-caju (10.9 mg g-1), while P. sajor-caju was more efficient in soluble protein production (40.2 mg g-1) compared to P. ostreatus (3.1 mg g-1). P. ostreatus was found to be more efficient in the individual components of cellulase enzyme production than P. sajor-caju. All the enzyme activities were found to attain a peak at 10 days of fermentation. The highest activities of endoglucanase, exoglucanase, and β-glucosidase .