tailieunhanh - A cellulase-free, thermoactive, and alkali xylanase production by terrestrial Streptomyces sp. CA24

Microbial xylanases have attracted much attention because of their applications in industrial processes during the past few decades. Sixty-five terrestrial actinomycetes previously isolated from rhizospheric soils of endemic plants were tested for their ability in production of xylanase. | Turkish Journal of Biology Research Article Turk J Biol (2013) 37: 370-375 © TÜBİTAK doi: A cellulase-free, thermoactive, and alkali xylanase production by terrestrial Streptomyces sp. CA24 İlknur PORSUK, Süleyman ÖZAKIN, Bülent BALİ, Ebru İNCE YILMAZ* Department of Biology, Faculty of Science, Dicle University, 21280 Diyarbakır, Turkey Received: Accepted: Published Online: Printed: Abstract: Microbial xylanases have attracted much attention because of their applications in industrial processes during the past few decades. Sixty-five terrestrial actinomycetes previously isolated from rhizospheric soils of endemic plants were tested for their ability in production of xylanase. The results revealed that 33 isolates could degrade xylan on both solid and liquid media. Extracellular xylanase production by these isolates was characterized during their growth in liquid medium. Streptomyces sp. CA24, one of the most active strains, was selected for optimization of xylanase production. The enzyme secretion was enhanced when the medium was supplemented with oat spelt xylan () and yeast extract () at pH . Optimization of the culture conditions resulted in a 5-fold enhancement in enzyme production (255 U/mL). The most important characteristic of the enzyme is its production over a broad pH range of – during all stationary phases. The cellulase-free xylanase was optimally active at 60 °C and almost 90% stable after 4 h at 50 °C. The high levels of enzyme production obtained under alkali batch cultivation conditions, coupled with no production of cellulase, suggests the use of the local terrestrial strain Streptomyces CA24 and/or its enzyme in industrial processes such as pulp and paper production. Key words: Streptomyces, xylanase, medium optimization, alkali-thermoactive enzyme 1. Introduction Xylanases are enzymes used in various industrial .

• Sinh học
• Medium optimization
• Alkali thermoactive enzyme
• Rhizospheric soils
• Cellulase free xylanase
• Xylanase producer actinomycetes
• Iron uptake
• Growth medium
• Growth medium optimization
• High production
• Iron chelator production potential
• BMC Biotechnology
• Aspergillus Niger
• Bioprocess optimization
• Batch culture
• Fed batch culture
• International Conference on Emerging Challenges
• Operations optimization
• Optimization software
• Small and medium sized enterprises (SMEs)
• Enterprise resource planning
• Inventory management
• Cunninghamella sp 2A1
• Response surface
• Response surface methodology
• Production of biomass
• Endophytic actinobacteria
• Streptomyces sp
• Fermentation medium
• Endophytic streptomyces
• Production of antibacterial compounds
• Lignocellulosics waste
• Millet straw
• Aspergillus flavus
• CMC agar medium
• Solid state fermentation
• Saccharomyces cerevisiae
• Antimicrobial activity
• Lactobacillus acidophilus
• Heat stability
• pH stability
• Storage stability
• Bacillus subtilis
• Cane molasses
• Rice bran
• FTIR spectrophotometry
• Biochemical characterization