Đang chuẩn bị liên kết để tải về tài liệu:
PCR detection of Fusarium oxysporum f. sp. radicis-lycopersici and races of F. oxysporum f. sp. lycopersici of tomato in protected tomato-growing areas of the eastern Mediterranean region of Turkey

Fusarium crown and root rot (caused by Fusarium oxysporum f. sp. radicis-lycopersici, FORL) and Fusarium wilt (caused by Fusarium oxysporum f. sp. lycopersici, FOL) are the most important diseases to affect tomatoes in protected growing conditions in the eastern Mediterranean region of Turkey. | Turkish Journal of Agriculture and Forestry http://journals.tubitak.gov.tr/agriculture/ Research Article Turk J Agric For (2013) 37: 457-467 © TÜBİTAK doi:10.3906/tar-1203-71 PCR detection of Fusarium oxysporum f. sp. radicis-lycopersici and races of F. oxysporum f. sp. lycopersici of tomato in protected tomato-growing areas of the eastern Mediterranean region of Turkey 1, 2 Ayşegül ÇOLAK *, Mehmet BİÇİCİ Biological Control Research Station, Adana, Turkey 2 Department of Plant Protection, Faculty of Agriculture, Çukurova University, Adana, Turkey 1 Received: 30.03.2012 Accepted: 22.12.2012 Published Online: 16.07.2013 Printed: 02.08.2013 Abstract: Fusarium crown and root rot (caused by Fusarium oxysporum f. sp. radicis-lycopersici, FORL) and Fusarium wilt (caused by Fusarium oxysporum f. sp. lycopersici, FOL) are the most important diseases to affect tomatoes in protected growing conditions in the eastern Mediterranean region of Turkey. These diseases cause significant yield losses in this region. Polymerase chain reaction (PCR) studies were used to characterize 87 isolates from Adana and Mersin provinces, representative of different locations. Among them, 60% and 40% of the plants were determined to have FORL and FOL, respectively. FORL and FOL race 3 are the dominant pathogens in this region. Some differences between morphological identification and molecular detection (PCR) were observed. Key words: Fusarium oxysporum f. sp. lycopersici, Fusarium oxysporum f. sp. radicis-lycopersici, PCR detection, tomato 1. Introduction In covered tomato-growing areas in the eastern Mediterranean region, it was previously known that the wilt disease caused by Fusarium oxysporum f. sp. lycopersici (Sacc.) W.C. Snyder & H.N. Hans (FOL) was of prime importance. Later, crown and root-rot diseases caused by F. oxysporum f. sp. radicis-lycopersici Jarvis & Shoemaker (FORL) were encountered more frequently (Yücel and Çınar 1989; Can et al. 2004). F. oxysporum has spread .