tailieunhanh - Rapid PCR based detection of buffalo milk in cow milk

The study was undertaken with an objective to evaluate a rapid PCR based method for detection of adulteration of buffalo milk in cow milk at minimum level of detection. This method utilizes primers targeting the mitochondrial encoded 12S rRNA gene as the target for species identification. PCR assay involve use of three different primers. Reverse primers specific for cow and buffalo complementary to the gene fragment of 12S rRNA along with the common forward primer. The cow specific primer, along with the common forward primer, yields a cow specific amplicon of 346 bp in the 12S rRNA gene. On the other hand, a buffalo specific primer along with the same common forward primer yields a buffalo specific amplicon of 220 bp fragment in the same gene. The method evaluated was able to detect presence of buffalo milk in cow at level of adulteration. | 2019 8 4 791-797 EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 8 Number 04 2019 Journal homepage http Original Research Article https Rapid PCR Based Detection of Buffalo Milk in Cow Milk . Khade . Maurya . Ebenezer . Patel A. Balakrishnan and Rajesh R. Nair National Dairy Development Board Anand Gujarat India Corresponding author ABSTRACT Keywords Cattle Buffalo Milk Adulteration PCR Keywords Cattle Buffalo Milk Adulteration PCR Article Info Accepted 07 March 2019 Available Online 10 April 2019 The study was undertaken with an objective to evaluate a rapid PCR based method for detection of adulteration of buffalo milk in cow milk at minimum level of detection. This method utilizes primers targeting the mitochondrial encoded 12S rRNA gene as the target for species identification. PCR assay involve use of three different primers. Reverse primers specific for cow and buffalo complementary to the gene fragment of 12S rRNA along with the common forward primer. The cow specific primer along with the common forward primer yields a cow specific amplicon of 346 bp in the 12S rRNA gene. On the other hand a buffalo specific primer along with the same common forward primer yields a buffalo specific amplicon of 220 bp fragment in the same gene. The method evaluated was able to detect presence of buffalo milk in cow at level of adulteration. Introduction In recent times with increasing consumption of dairy food items the species identification of milk and milk products has received a great attention. It has a significant importance for several reasons related to government regulations religion and public health. The extensive consumption of milk and dairy products makes these foodstuffs targets for potential adulteration with financial gains for unscrupulous producers Nicolaou et al. 2011 . In the dairy sector the .