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Purification and partial characterization of intracellular fructosyltransferase from a novel strain of Aureobasidium pullulans

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The intracellular fructosyltransferase (FTase) of a novel strain of Aureobasidium pullulans (CFR 77) capable of producing 59% of fructooligosaccharides (FOS) within 9 h of reaction time was obtained by wet-milling, and then purified and characterized. | Turk J Biol 31 (2007) 147-154 © TÜB‹TAK Purification and Partial Characterization of Intracellular Fructosyltransferase from a Novel Strain of Aureobasidium pullulans Agbaje LATEEF1, Julius Kola OLOKE1, Siddalingaiya G. PRAPULLA2 1 Department of Pure and Applied Biology, Ladoke Akintola University of Technology, PMB 4000, Ogbomoso - NIGERIA 2 Department of Fermentation Technology and Bioengineering, Central Food Technological Research Institute, Mysore 570 020 - INDIA Received: 01.12.2006 Abstract: The intracellular fructosyltransferase (FTase) of a novel strain of Aureobasidium pullulans (CFR 77) capable of producing 59% of fructooligosaccharides (FOS) within 9 h of reaction time was obtained by wet-milling, and then purified and characterized. The purified FTase revealed 2 bands of 147 and 170 KD; its activity was optimum at an approximate pH of 5.0 and temperature of 55 °C. The specific activity of the final purified material was 42, representing a purification factor of 79.44 and yield of 43%. The enzyme is very stable, retaining more than 80% of its original activity at the optimum reaction conditions after 12 h. Using the crude intracellular FTase, 59% of FOS was produced within 9 h of reaction time, which is a considerable reduction in the reaction time of 12-25 h that has been reported in the literature. The purified FTase yielded 59% of FOS within 3 h of reaction time. Key Words: Fructosyltransferase, Aureobasidium pullulans, enzyme purification, fructooligosaccharides Aureobasidium pullulans Suflundan Hücreiçi Fruktoziltransferaz Enziminin Saflaflt›r›lmas› ve K›smi Karekterizasyonu Özet: Dokuz saatlik reaksiyon süresinde %59 fructosiltransferaz üretebilen yeni bir Aureobasidium pullulans (CFR77) suflunun hücre içi FOS’u wet-milling yöntemi ile elde edilmifl, saflaflt›r›lm›fl ve karakterize edilmifltir. Saflaflt›r›lan FTaz 147 ve 170 KD a¤›rl›¤›nda iki bant vermifltir, aktivitesi yaklafl›k pH 5 ve 55 °C’de optimum olarak bulunmufltur. Sonuçta saflaflt›r›lan .

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