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A comparison of germ tube production by Candida albicans in three media
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The conversion of C. albicans. from commensal to the pathogenic state is associated with its ability to make the dimorphic transition from the yeast to the hyphal (germ tube) form. Three liquid media were compared in ability to promote germ tube production by C. albicans. | Tr. J. of Biology 22 (1998) 205-210 © TÜBİTAK A Comparison of Germ Tube Production by Candida albicans in Three Media Gülhan VARDAR-ÜNLÜ Department of Microbiology, Faculty of Medicine, University of Cumhuriyet 58140, Sivas - TURKEY Received: 25.06.1997 Abstract: The conversion of C. albicans. from commensal to the pathogenic state is associated with its ability to make the dimorphic transition from the yeast to the hyphal (germ tube) form. Three liquid media were compared in ability to promote germ tube production by C. albicans. Medium 199 and Glucose-Glycine Medium at 37˚C were highly satisfactory and gave 90 % conversion of yeast cells to hyphal-form cells. Sucrose-Gelatine medium gave only 15% conversion. Glucose-Glycine medium appeared to give more rapid germ tube production than 199 Medium. Key Words: Candida albicans, germ tube, medium Üç Ortamda Candida albicans 'ın Germ Tüp Oluşumunun Kıyaslanması Özet: C. albicans' ın komersal durumdan pathojenik duruma dönüsümü, maya formundan hif (jerm tüp) formuna dimorfik geçişi yapılabilmesiyle ilgilidir. Üç sıvı ortamda Candida albicans 'ın germ tüp oluşumu uyarabilmesi için karşılaştırıldı. 199 Besiyeri ve Glukoz-Glisin Besiyeri 37˚C de oldukça başarılı sonuçlar vermiş olup, % 90 oranında maya formundan hif formuna dönüşümünü sağladı. SukrozJelatin Besiyeri sadece %15'lik dönüşüm sağladı. Glukoz-Glisin Besiyeri, 199 Besiyerin'den daha hızlı germ tüp oluşturdu. Anahtar Sözcükler: C.albicans, germ tüp, besiyeri Introduction C. albicans is a opportunistic pathogen and is dimorphic. The conversion of C. albicans from the commensal to the pathogenic state is associated with transition from the yeast cell to the hyphal form (1), although the evidence in support of this is equivocal (2, 3). This phenomenon of dimorphism must involve reorganization of the cell wall at the molecular level as revealed by detection of antigens and adhesins on hyphal-form cells (4-9). The morphological development of C. albicans can be