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Genetic variability in Grapevine virus A from Vitis vinifera L. × Vitis labrusca L. in Sichuan, China
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Grapevine virus A (GVA) has a quasispecies nature and is closely associated with the rugose-wood disease complex of Vitis vinifera L. In the diff erent growing regions of table grape in Sichuan, southwest China, the incidence of GVA infection was found to be 16.9% by ELISA detection among 178 grape plants (Vitis vinifera L. × Vitis labrusca L.). | Turk J Biol 36 (2012) 542-551 © TÜBİTAK doi:10.3906/biy-1203-23 Genetic variability in Grapevine virus A from Vitis vinifera L. × Vitis labrusca L. in Sichuan, China Jianhui WANG1,2,3, Dehui XI1, Jianjun LIU3,4, Keling CHEN2,3, Hongwen LI2,3, Xiao LIU2,3, Shu YUAN1, Sezai ERCİŞLİ5, Honghui LIN1 1 Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064 - P.R. CHINA 2 Horticulture Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066 - P.R. CHINA 3 Key Laboratory of Horticultural Crops of Biology and Genetic Improvement (Southwest Region), Ministry of Agriculture, Chengdu 610066 - P.R. CHINA 4 Sichuan Academy of Agricultural Sciences, Chengdu 610066 - P.R. CHINA 5 Department of Horticulture, Faculty of Agriculture, Atatürk University, 25240 Erzurum - TURKEY Received: 14.03.2012 ● Accepted: 10.04.2012 Abstract: Grapevine virus A (GVA) has a quasispecies nature and is closely associated with the rugose-wood disease complex of Vitis vinifera L. In the different growing regions of table grape in Sichuan, southwest China, the incidence of GVA infection was found to be 16.9% by ELISA detection among 178 grape plants (Vitis vinifera L. × Vitis labrusca L.). The results of restriction fragment length polymorphism (RFLP) studies on the polymerase chain reaction (PCR) products of a total of 139 plasmids, cloned from 15 GVA isolates, suggested that the GVA isolates contained highly divergent variants. The plasmids from each GVA isolate that yielded different PCR-RFLP profiles were preferentially chosen for sequencing and were designated as variants. Furthermore, a phylogenetic study based on the analysis of the GVA coat protein genes and RNA silencing suppressor genes showed that the 40 variants obtained from the 15 GVA isolates represented 4 clades, designated as molecular groups I, II, III, and IV, respectively. The variant-specific PCR detection results indicated that .