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Improved Chitin and Chitosan Production from Black Tiger Shrimp Shells Using Salicylic Acid Pretreatment

Abstract: Shrimp chitin and chitosan with improved characteristics were isolated from black tiger shrimp waste pretreated with 0.04 M Salicylic acid for ten hours. The pretreated shrimp shells could be efficiently demineralized and deproteinized at ambient temperature using 0.680 M HCl and 0.620 M NaOH, respectively. The duration of the treatments was 8 hours, the ash and protein residues in the final chitosan were about 0.48% and 0.51% respectively; the viscosity was 4800 cps; the solubility was up to 98%. In comparison with treatment at ambient temperature (30oC) without pretreatment, the chemical consumption, the duration of the treatment, ash and protein residues was reduced to. | The Open Biomaterials Journal 2011 3 1-3 1 Open Access Improved Chitin and Chitosan Production from Black Tiger Shrimp Shells Using Salicylic Acid Pretreatment Nguyen Van Toan School of Biotechnology International University Ho Chi Minh City Vietnam Vietnam National University Ho Chi Minh City Vietnam Abstract Shrimp chitin and chitosan with improved characteristics were isolated from black tiger shrimp waste pretreated with 0.04 M Salicylic acid for ten hours. The pretreated shrimp shells could be efficiently demineralized and deproteinized at ambient temperature using 0.680 M HCl and 0.620 M NaOH respectively. The duration of the treatments was 8 hours the ash and protein residues in the final chitosan were about 0.48 and 0.51 respectively the viscosity was 4800 cps the solubility was up to 98 . In comparison with treatment at ambient temperature 30oC without pretreatment the chemical consumption the duration of the treatment ash and protein residues was reduced to 75-20 whereas viscosity and absence of insolubles improved by a factor of 2.5. Keywords Chitin chitosan membrane pretreated Salicylic acid wound healing management. INTRODUCTION Chitin and chitosan are the structural components in the cuticles of crustacean insects mollusk and in the cell wall of fungi and plant pathogens Suwalee 2002 1 . Reportedly chitin and chitosan have various biological functions for instance antimicrobial activity wound healing especially on the proliferative phase and matrix formation. Since the early eighties chitin and its derivatives have been used for various biomedical purposes especially in skin and wound management products in Japan 2 . Though several standard chemical procedures have been widely used to produce chitin and chitosan utilizing shrimp shell waste as raw material there is not much information about how to improve the production process so as to produce chitosan with consistent quality especially high solubility high viscosity and high stability in degree of