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Báo cáo khoa học: O-MACS, a novel member of the medium-chain acyl-CoA synthetase family, specifically expressed in the olfactory epithelium in a zone-specific manner
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In rodents, the olfactory epithelium(OE) canbe divided into four topographically distinct zones, and eachmember of the odorant receptor(OR) gene family is expressed only in one particular zone. To study the functional significance of the zonal structure of the OE, we searched for genes expressed in a zone-specific manner by using the differential display method. Among the clones isolated from the rat OE, we characterized a novel olfactory protein termed O-MACS, a member of the medium-chain acyl-CoA synthetase family | Eur. J. Biochem. 270 1995-2004 2003 FEBS 2003 doi 10.1046 j.1432-1033.2003.03571.x O-MACS a novel member of the medium-chain acyl-CoA synthetase family specifically expressed in the olfactory epithelium in a zone-specific manner Yuichiro Oka Ko Kobayakawa Hirofumi Nishizumi Kazunari Miyamichi Satoshi Hirose Akio Tsuboi and Hitoshi Sakano Department of Biophysics and Biochemistry Graduate School of Science The University of Tokyo CREST Program of Japan Science and Technology Corporation Japan In rodents the olfactory epithelium OE can be divided into four topographically distinct zones and each member of the odorant receptor OR gene family is expressed only in one particular zone. To study the functional significance of the zonal structure of the OE we searched for genes expressed in a zone-specific manner by using the differential display method. Among the clones isolated from the rat OE we characterized a novel olfactory protein termed O-MACS a member of the medium-chain acyl-CoA synthetase family. The o-macs gene encodes a protein of 580 amino acids sharing 56-63 identity with other MACS family proteins. RT-PCR analysis demonstrated that the o-macs gene is expressed only in the OE unlike other MACS family genes. In situ hybridization revealed that the o-macs transcripts are present in the neuronal cell layer of olfactory sensory neurons OSNs as well as in the supporting and basal cell layers in the most dorso-medial area zone 1 of the OE. Developmental analysis revealed that the o-macs gene is already expressed on embryonic day 11.5 before the onset of the OR gene expression in a restricted area within the rat olfactory placode. Recombinant O-MACS protein tagged with c-Myc and His6 demonstrated an acyl-CoA synthetase activity for fatty acid activation and protein localization to mitochondria like other MACS family proteins. The present study indicates that this novel protein may play important roles in processing odorants in a zone-specific manner or the zonal .