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Báo cáo khoa học: Fatty acid composition of chylomicron remnant-like particles influences their uptake and induction of lipid accumulation in macrophages

The influence of the fatty acid composition of chylomicron remnant-like particles (CRLPs) on their uptake and induction of lipid accumulation in macrophages was studied. CRLPs containing triacylglycerol enriched in saturated, monounsaturated, n)6or n)3 polyunsaturated fatty acids derived from palm, olive, corn or fish oil, respectively, and macrophages derived from the human monocyte cell line THP-1 were used. | ễFEBS Journal Fatty acid composition of chylomicron remnant-like particles influences their uptake and induction of lipid accumulation in macrophages Clara De Pascale1 Michael Avella1 Javier S. Perona2 Valentina Ruiz-Gutierrez2 Caroline P. D. Wheeler-Jones1 and Kathleen M. Botham1 1 Department of Veterinary Basic Sciences RoyalVeterinary College London UK 2 Instituto de la Grasa CSIC Seville Spain Keywords chylomicron remnant-like particles fatty acid composition lipid accumulation macrophages Correspondence K. M. Botham Department of Veterinary Basic Sciences RoyalVeterinary College RoyalCollege St London NW1 0TU UK Fax 44 207468 5204 Tel 44 207468 5274 E-mail kbotham@rvc.ac.uk Received 30 August 2006 revised 10 October 2006 accepted 23 October 2006 doi 10.1111 j.1742-4658.2006.05552.x The influence of the fatty acid composition of chylomicron remnant-like particles CRLPs on their uptake and induction of lipid accumulation in macrophages was studied. CRLPs containing triacylglycerol enriched in saturated monounsaturated n-6 or n-3 polyunsaturated fatty acids derived from palm olive corn or fish oil respectively and macrophages derived from the human monocyte cell line THP-1 were used. Lipid accumulation triacylglycerol and cholesterol in the cells was measured after incubation with CRLPs for 5 24 and 48 h and uptake over 24 h was determined using CRLPs radiolabelled with 3H triolein. Total lipid accumulation in the macrophages was significantly greater with palm CRLPs than with the other three types of particle. This was mainly due to increased triacylglycerol concentrations whereas changes in cholesterol concentrations did not reach significance. There were no significant differences in lipid accumulation after incubation with olive corn or fish CRLPs. Palm and olive CRLPs were taken up by the cells at a similar rate which was considerably faster than that observed with corn and fish CRLPs. These findings demonstrate that CRLPs enriched in saturated or .