tailieunhanh - báo cáo khoa học: " Characterization and analysis of the cotton cyclopropane fatty acid synthase family and their contribution to cyclopropane fatty acid synthesis"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Characterization and analysis of the cotton cyclopropane fatty acid synthase family and their contribution to cyclopropane fatty acid synthesis | Yu et al. BMC Plant Biology 2011 11 97 http 1471-2229 11 97 BMC Plant Biology RESEARCH ARTICLE Open Access Characterization and analysis of the cotton cyclopropane fatty acid synthase family and their contribution to cyclopropane fatty acid synthesis Xiao-Hong Yu1 Richa Rawat1 and John Shanklin1 2 Abstract Background Cyclopropane fatty acids CPA have been found in certain gymnosperms Malvales Litchi and other Sapindales. The presence of their unique strained ring structures confers physical and chemical properties characteristic of unsaturated fatty acids with the oxidative stability displayed by saturated fatty acids making them of considerable industrial interest. While cyclopropenoid fatty acids CPE are well-known inhibitors of fatty acid desaturation in animals CPE can also inhibit the stearoyl-CoA desaturase and interfere with the maturation and reproduction of some insect species suggesting that in addition to their traditional role as storage lipids CPE can contribute to the protection of plants from herbivory. Results Three genes encoding cyclopropane synthase homologues GhCPS1 GhCPS2 and GhCPS3 were identified in cotton. Determination of gene transcript abundance revealed differences among the expression of GhCPS1 2 and 3 showing high intermediate and low levels respectively of transcripts in roots and stems whereas GhCPS1 and 2 are both expressed at low levels in seeds. Analyses of fatty acid composition in different tissues indicate that the expression patterns of GhCPS1 and 2 correlate with cyclic fatty acid CFA distribution. Deletion of the N-terminal oxidase domain lowered GhCPS s ability to produce cyclopropane fatty acid by approximately 70 . GhCPS1 and 2 but not 3 resulted in the production of cyclopropane fatty acids upon heterologous expression in yeast tobacco BY2 cell and Arabidopsis seed. Conclusions In cotton GhCPS1 and 2 gene expression correlates with the total CFA content in roots stems and seeds. That GhCPS1 and 2 .