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Báo cáo khóa học: Spatio-temporal distribution of cellular retinol-binding protein gene transcripts (CRBPI and CRBPII ) in the developing and adult 1 zebrafish (Danio rerio)

We have cloned and determined the nucleotide sequence of the cDNAcoding for a cellular retinol-bindingprotein type I (CRBPI)from zebrafish. The deduced amino acid sequence of the zebrafish CRBPI showed highest sequence identity ( 59%)to the mammalian CRBPIs of the intracellular lipid-binding protein (iLBP)multigene family. Phylogenetic analysis clustered the zebrafish CRBPI to the CRBPI clade. The zebrafish CRBPI gene (rbp1)and CRBPII gene (rbp2) both consist of four exons separated by three introns, iden-tical to all otheriLBP genes in vertebrates | Eur. J. Biochem. 271 339-348 2004 FEBS 2003 doi 10.1046 j.1432-1033.2003.03932.x Spatio-temporal distribution of cellular retinol-binding protein gene transcripts CRBPIand CRBPII in the developing and adult zebrafish Danio rerio Rong-Zong Liu1 Eileen M. Denovan-Wright2 Agnes Degrave3 Christine Thisse3 Bernard Thisse3 and Jonathan M. Wright1 1 Department of Biology and department of Pharmacology Dalhousie University Halifax Nova Scotia Canada 3Institut de Génétique et Biologie Moleculaire et Cellulaire Department of Developmental Biology UMR 7104 CNRS INSERM ULP Illkirch France We have cloned and determined the nucleotide sequence of the cDNA coding for a cellular retinol-binding protein type I CRBPI from ebrníìsh. The ded uced amiino acid eeq Lienee of the zebrahsh CRBPI showed highest sequence identity w 59 to the marmnalian CRBPE of the intrceellular lipid-binding protein iLBP m Ll 1 ii gene aaailly. Phylogeneiie analysis clustered the zebrahsh CRBPI to the CRBPI clade. The zebrahsh CRBPI gene rbpl and CRBPII i ie rbp2 both consist of four exons separated by three introns identical to all other iLBP genes in vertebrates. Two transcription start sites were identihed in the rbpl promoter and a single transcription start site was identihed for rbp2. Radiation hybrid mapping assigned the zebrahsh rbpl gene to linkage group 16 and conserved syntenic genes were found by comparative analysis of mammalian orthologous rbpl genes. RT-PCR detected mRNA transcripts in the adult intestine liver brain ovary and testis for rbpl gene and in the intestine and liver for rbp2 gene. Whole mount in situ hybridization of zebrahsh embryos revealed rbpl mRNA expression in the developing zebrahsh central nervous system at specihc sites that are known to have abundant retinoic acid distribution and sigmhcant retinoic acid action. Whole mount in situ hybridization also showed that the zebrahsh rbp2 mRNA was localized specihcally in the embryonic intestinal bulb and the developing intestine