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Báo cáo khoa học: Sulfated polysaccharides inhibit the catabolism and loss of both large and small proteoglycans in explant cultures of tendon

This study investigated the effects of two highly sulfated polysaccharides, calcium pentosan polysulfate and heparin, on the loss of newly synthesized proteoglycans from the matrix of explant cultures of bovine tendon. The tensional region of deep flexor tendon was incubated with [ 35 S]sulfate for 6 h and then placed in culture for up to 15 days. | ềFEBS Journal Sulfated polysaccharides inhibit the catabolism and loss of both large and small proteoglycans in explant cultures of tendon Tom Samiric Mirna Z. Ilic and Christopher J. Handley Schoolof Human Biosciences La Trobe University Melbourne Australia Keywords aggrecan calcium pentosan polysulfate decorin heparin proteoglycan tendon Correspondence T. Samiric School of Human Biosciences La Trobe University 3086 Victoria Australia Fax 61 394795784 Tel 61 394793417 E-mail T.Samiric@latrobe.edu.au Received 10 May 2006 accepted 2 June 2006 doi 10.1111 j.1742-4658.2006.05348.x This study investigated the effects of two highly sulfated polysaccharides calcium pentosan polysulfate and heparin on the loss of newly synthesized proteoglycans from the matrix of explant cultures of bovine tendon. The tensional region of deep flexor tendon was incubated with 35S sulfate for 6 h and then placed in culture for up to 15 days. The amount of radiolabel associated with proteoglycans lost to the medium and retained in the matrix was determined for each day in culture. It was shown that both sulfated polysaccharides at concentrations of 1000 Ltg-mL 1 inhibited the loss of 35S-labeled large and small proteoglycans from the matrix and concomitant with this was a retention of chemical levels of proteoglycans in the explant cultures. In other explant cultures that were maintained in culture in the presence of both agents for more than 5 days after incubation with 35S sul-fate inhibition of the intracellular catabolic pathway was evident indicating that these highly sulfated polysaccharides also interfered with the intracellular uptake of small proteoglycans by tendon cells. The role of tendons is to absorb and transmit force generated by muscles to bones the basis of joint movement. Tendons are fibrous connective tissues that are sparsely populated with cells that are responsible for the synthesis and maintenance of the extracellular matrix. Type I collagen is the major macromolecule