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Báo cáo khoa học: Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria

Soluble inorganic pyrophosphatases (inorganic diphosphatases, EC 3.6.1.1) were isolated and characterized from three phylogenetically diverse cyano-bacteria)Synechocystis sp. PCC 6803, Anabaena sp. PCC 7120, and Pseudanabaenasp. PCC 6903 – and one anoxygenic photosynthetic bacter-ium, Rhodopseudomonas viridis(purple nonsulfur). | ễFEBS Journal Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria Maria R. Gomez-Garcia Manuel Losada and Aurelio Serrano Institute de Bioquimica Vegetaly Fotosintesis Centro de Investigaciones Cientificas Isla Cartuja CSIC-Universidad de Sevilla Spain Keywords anoxygenic photosynthetic bacteria cyanobacteria functional complementation ppa soluble pyrophosphatases Correspondence M. R. Gomez-Garcia Department of Biochemistry Stanford University Schoolof Medicine Beckman Center B413 300 Pasteur Dr. Stanford CA 94305-5307 USA Fax 1 650 725 6044 Tel 1 650 723 5348 E-mail mrgomez@stanford.edu A. Serrano Instituto de Bioquimica Vegetal y Fotosintesis CSIC-Univ. de Sevilla Avda. Americo Vespucio 49 41092 - Sevilla Spain Fax 34 954460065 Tel 34 954489524 E-mail aurelio@ibvf.csic.es Present address Department of Biochemistry Stanford University School of Medicine Beckman Center B413 Stanford CA USA Received 17 April2007 revised 23 May 2007 accepted 8 June 2007 doi 10.1111 j.1742-4658.2007.05927.x Soluble inorganic pyrophosphatases inorganic diphosphatases EC 3.6.1.1 were isolated and characterized from three phylogenetically diverse cyanobacteria - Synechocystis sp. PCC 6803 Anabaena sp. PCC 7120 and Pseudanabaena sp. PCC 6903 - and one anoxygenic photosynthetic bacterium Rhodopseudomonas viridis purple nonsulfur . These enzymes were found to be family I soluble inorganic pyrophosphatases with c. 20 kDa subunits with diverse oligomeric structures. The corresponding ppa genes were cloned and functionally validated by heterologous expression. Cyano-bacterial family I soluble inorganic pyrophosphatases were strictly Mg2 -dependent enzymes. However diverse cation cofactor dependence was observed for enzymes from other groups of photosynthetic bacteria. Immunochemical studies with antibodies to cyanobacterial soluble inorganic pyrophosphatases showed crossreaction with orthologs of other main groups of .