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Báo cáo khoa học: Utp25p, a nucleolar Saccharomyces cerevisiae protein, interacts with U3 snoRNP subunits and affects processing of the 35S pre-rRNA

In eukaryotes, pre-rRNA processing depends on a large number of nonribo-somaltrans-acting factors that form intriguingly organized complexes. Two intermediate complexes, pre-40Sand pre-60S, are formed at the early stages of 35Spre-rRNA processing and give rise to the mature ribosome subunits. Each of these complexes contains specific pre-rRNAs, some ribosomal proteins and processing factors. | Utp25p a nucleolar Saccharomyces cerevisiae protein interacts with U3 snoRNP subunits and affects processing of the 35S pre-rRNA Mauricio B. Goldfeder and Carla C. Oliveira Department of Biochemistry University of Sao Paulo SP Brazil Keywords nucleolus pre-40S ribosome synthesis rRNA processing Saccharomyces cerevisiae Correspondence C. C. Oliveira Department of Biochemistry Chemistry Institute University of Sao Paulo Av. Prof. Lineu Prestes 748 Scio Paulo SP BrazilCEP 05508-900 Fax 55 11 3815 5579 Tel 55 11 3091 3810 Ext. 208 E-mail ccoliv@iq.usp.br Received 19 November 2009 revised 31 March 2010 accepted 28 April 2010 doi 10.1111 j.1742-4658.2010.07701.x In eukaryotes pre-rRNA processing depends on a large number of nonribo-somal trans-acting factors that form intriguingly organized complexes. Two intermediate complexes pre-40S and pre-60S are formed at the early stages of 35S pre-rRNA processing and give rise to the mature ribosome subunits. Each of these complexes contains specific pre-rRNAs some ribosomal proteins and processing factors. The novel yeast protein Utp25p has previously been identified in the nucleolus an indication that this protein could be involved in ribosome biogenesis. Here we show that Utp25p interacts with the SSU processome proteins Sas10p and Mpp10p and affects 18S rRNA maturation. Depletion of Utp25p leads to accumulation of the pre-rRNA 35S and the aberrant rRNA 23 S and to a severe reduction in 40S ribosomal subunit levels. Our results indicate that Utp25p is a novel SSU processome subunit involved in pre-40S maturation. Structured digital abstract MINT-7889901 SAS10 uniprotkb Q12136 physically interacts MI 0915 with Utp25p uni-protkb P40498 by pull down MI 0096 MINT-7889915 NIP7 uniprotkb Q08962 physically interacts MI 0915 with RRP43 uni-protkb P25359 by two hybrid MI 0018 MINT-7889852 Utp25p uniprotkb P40498 physically interacts MI 0915 with MPP10 uniprotkb P47083 by two hybrid MI 0018 MINT-7890065 NOP1 uniprotkb P15646 and Utp25p