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THE DNA SEQUENCING – METHODS AND APPLICATIONS

Semi-intact cells are cells whose plasma membrane has been permeabilized with detergent or toxins, and can be referred to as “cell-type test tubes” (Fig. 1). We use a bacterial poreforming toxin, streptolysin O (SLO), to permeabilize the cells. At 4℃, SLO binds to cholesterol in plasma membranes. At warmer temperatures, SLO assembles to form amphiphilic oligomers, which results in the generation of small, stable transmembrane pores (Bhakdi et al., 1985). SLO-induced pores are approximately 30 nm in diameter, which is sufficiently large to allow immunoglobulin to enter into the cells (immunoglobulin G: 150 kDa). Protein complexes that are larger than immunoglobulin, such as homo- or heterooligomers, can enter. | DNA SEQUENCING -METHODS AND APPLICATIONS Edited by Anjana Munshi INTECHOPEN.COM DNA Sequencing - Methods and Applications Edited by Anjana Munshi Published by InTech Janeza Trdine 9 51000 Rijeka Croatia Copyright 2012 InTech All chapters are Open Access distributed under the Creative Commons Attribution 3.0 license which allows users to download copy and build upon published articles even for commercial purposes as long as the author and publisher are properly credited which ensures maximum dissemination and a wider impact of our publications. After this work has been published by InTech authors have the right to republish it in whole or part in any publication of which they are the author and to make other personal use of the work. Any republication referencing or personal use of the work must explicitly identify the original source. As for readers this license allows users to download copy and build upon published chapters even for commercial purposes as long as the author and publisher are properly credited which ensures maximum dissemination and a wider impact of our publications. Notice Statements and opinions expressed in the chapters are these of the individual contributors and not necessarily those of the editors or publisher. No responsibility is accepted for the accuracy of information contained in the published chapters. The publisher assumes no responsibility for any damage or injury to persons or property arising out of the use of any materials instructions methods or ideas contained in the book. Publishing Process Manager Bojan Rafaj Technical Editor Teodora Smiljanic Cover Designer InTech Design Team First published April 2012 Printed in Croatia A free online edition of this book is available at www.intechopen.com Additional hard copies can be obtained from orders@intechopen.com DNA Sequencing - Methods and Applications Edited by Anjana Munshi p. cm. ISBN 978-953-51-0564-0 INTECH open science I open minds free online editions of InTech Books and .