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Báo cáo y học: "Histone deacetylase inhibitor, butyrate, attenuates lipopolysaccharide-induced acute lung injury in mice"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"Histone deacetylase inhibitor, butyrate, attenuates lipopolysaccharide-induced acute lung injury in mice. | Ni et al. Respiratory Research 2010 11 33 http respiratory-research.eom content 11 1 33 RESPIRATORY RESEARCH RESEARCH Open Access Histone deacetylase inhibitor butyrate attenuates lipopolysaccharide-induced acute lung injury in mice Yun-Feng Ni Jian Wang Xiao-Long Yan Feng Tian Jin-Bo Zhao Yun-Jie Wang Tao Jiang Abstract Background Histone deacetylase HDAC inhibitors developed as promising anti-tumor drugs exhibit their antiinflammatory properties due to their effects on reduction of inflammatory cytokines. Objective To investigate the protective effect of butyrate a HDAC inhibitor on lipopolysaccharide LPS -induced acute lung injury ALI in mice. Methods ALI was induced in Balb c mice by intratracheally instillation of LPS 1 mg kg . Before 1 hour of LPS administration the mice received butyrate 10 mg kg orally. The animals in each group were sacrificed at different time point after LPS administration. Pulmonary histological changes were evaluated by hematoxylin-eosin stain and lung wet dry weight ratios were observed. Concentrations of interleukin IL -1p and tumor necrosis factor TNF -a in bronchoalveolar lavage fluid BALF and concentrations of nitric oxide NO and myeloperoxidase MPO activity in lung tissue homogenates were measured by enzyme-linked immunosorbent assay ELISA . Expression of nuclear factor NF -kB p65 in cytoplasm and nucleus was determined by Western blot analysis respectively. Results Pretreatment with butyrate led to significant attenuation of LPS induced evident lung histopathological changes alveolar hemorrhage and neutrophils infiltration with evidence of reduced MPO activity. The lung wet dry weight ratios as an index of lung edema were reduced by butyrate administration. Butyrate also repressed the production of TNF-a IL-1P and NO. Furthermore the expression of NF-kB p65 in nucleus was markedly suppressed by butyrate pretreatment. Conclusions Butyrate had a protective effect on LPS-induced ALI which may be related to its effect on suppression