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báo cáo khoa học: "Hepatocyte growth factor incorporated chitosan nanoparticles augment the differentiation of stem cell into hepatocytes for the recovery of liver cirrhosis in mice"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Hepatocyte growth factor incorporated chitosan nanoparticles augment the differentiation of stem cell into hepatocytes for the recovery of liver cirrhosis in mice | Pulavendran et al. Journal of Nanobiotechnology 2011 9 15 http www.jnanobiotechnology.eom content 9 1 15 JOURNAL OF NANOBIOTECHNOLOGY RESEARCH Open Access Hepatocyte growth factor incorporated chitosan nanoparticles augment the differentiation of stem cell into hepatocytes for the recovery of liver cirrhosis in mice Sivasami Pulavendran1 Chellan Rose1 and Asit Baran Mandal2 Abstract Background Short half-life and low levels of growth factors in the niche of injured microenvironment necessitates the exogenous and sustainable delivery of growth factors along with stem cells to augment the regeneration of injured tissues. Methods Here recombinant human hepatocyte growth factor HGF was incorporated into chitosan nanoparticles CNP by ionic gelation method and studied for its morphological and physiological characteristics. Cirrhotic mice received either hematopoietic stem cells HSC or mesenchymal stemcells MSC with or without HGF incorporated chitosan nanoparticles HGF-CNP and saline as control. Biochemical histological immunostaining and gene expression assays were carried out using serum and liver tissue samples. One way analysis of variance was used for statics application Results Serum levels of selected liver protein and enzymes were significantly increased in the combination of MSC and HGF-CNP MSC HGF-CNP treated group. Immunopositive staining for albumin Alb and cytokeratin 18 CK18 and reverse transcription-polymerase chain reaction RT-PCR for Alb alpha fetoprotein AFP CK18 cytokeratin 19 CK19 ascertained that MSC-HGF-CNP treatment could be an effective combination to repopulate liver parenchymal cells in the liver cirrhosis. Zymogram and western blotting for matrix metalloproteinases 2 and 9 MMP2 and MMP9 revealed that MMP2 actively involved in the fibrolysis of cirrhotic tissue. Immunostaining for alpha smooth muscle actin aSMA and type I collagen showed decreased expression in the MSC HGF-CNP treatment. These results indicated that HGF-CNP enhanced the .