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báo cáo khoa học: "Application of in situ reverse trancriptase-polymerase chain reaction (RT-PCR) to tissue microarrays"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Application of in situ reverse trancriptase-polymerase chain reaction (RT-PCR) to tissue microarrays | Journal of Nanobiotechnology BioMed Central Research Open Access Application of in situ reverse trancriptase-polymerase chain reaction RT-PCR to tissue microarrays Alasdair C Stamps Jonathan A Terrett and Paul J Adam Address Oxford GlycoSciences UK Ltd. The Form 86 Milton Park Abingdon UK OX14 4RY Email Alasdair C Stamps - astamps@ntlworld.com Jonathan ATerrett - jon.terrett@ogs.co.uk Paul J Adam - paul.adam@ogs.co.uk Corresponding author Published 28 May 2003 Received 29 April 2003 Accepted 28 May 2003 Journal of Nanobiotechnology 2003 1 3 This article is available from http www.jnanobiotechnology.cOm content 1 1 3 2003 Stamps et al licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Detection of disease-associated gene transcripts in primary disease tissues is frequently confounded by the presence of non-involved cell types. Alternative methods of detecting gene expression directly within tissues involve either the generation of antibodies which can be a lengthy process and may suffer from lack of specificity or amplification of reverse-transcribed cDNA in tissue sections in situ RT-PCR . The latter method is highly specific and enables detection of transcripts in the cells originally responsible for their synthesis but is highly destructive of tissue structures and can be carried out on only one or a few sections per experiment resulting in low reproducibility. In this study in situ RT-PCR was applied for the first time to commercially available tissue section microarrays enabling the examination of up to 70 different samples simultaneously. Modifications to the technique are detailed that preserved visible tissue and cellular structures and improved transcript detection whilst preventing significant generation of artefacts. Background Prior to the advent of miniaturisation the .