Đang chuẩn bị liên kết để tải về tài liệu:
Báo cáo khoa học: Binase cleaves cellular noncoding RNAs and affects coding mRNAs
Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ
Tải xuống
One of the least recognized causes of cellular damage duringex vivopreser-vation of red blood cells is oxidative injury to the hemoglobin. The latter has been associated with hemolysis through the release of toxic substances and oxidation of vital cell components. | Binase cleaves cellular noncoding RNAs and affects coding mRNAs Vladimir A. Mitkevich1 Nickolai A. Tchurikov1 Pavel V. Zelenikhin2 Irina Yu. Petrushanko1 Alexander A. Makarov1 and Olga N. Ilinskaya1 2 1 Engelhardt Institute of Molecular Biology Russian Academy of Sciences Moscow Russia 2 Department of Microbiology Kazan State University Kazan Russia Keywords apoptosis cellular RNA degradation cytotoxicity gene expression RNase Correspondence A. A. Makarov or O. N. Ilinskaya Engelhardt Institute of Molecular Biology Russian Academy of Sciences Moscow Russia Fax 7 499 1351405 Tel 7 499 1354095 E-mail aamakarov@eimb.ru olga.ilinskaya@ksu.ru Received 28 July 2009 revised 8 October 2009 accepted 2 November 2009 doi 10.1111 j.1742-4658.2009.07471.x Bacterial RNases are promising tools for the development of anticancer drugs. Neoplastic transformation leads to enhanced accumulation of rRNA and tRNA and altered expression of regulatory noncoding RNAs. Cleavage of RNA in cancer cells is the main reason for the cytotoxic effects of exogenic RNases. We have shown that binase a cytotoxic ribonuclease from Bacillus intermedius affects the total amount of intracellular RNA and the expression of proapoptotic and antiapoptotic mRNAs. For four cell lines we visualized cellular RNA by fluorescence microscopy and determined RNA levels viability and apoptosis by flow cytometry. We found that the level of cellular RNA was decreased in cells that were sensitive to the cytotoxic effects of binase. The RNA level was lowered by 44 in HEK cells transfected with the hSK4 gene of the Ca2 -activated potassium channels HEKhSK4 and by 20 in kit-transformed myeloid progenitor FDC-P1iR1171 cells. The most significant decrease in RNA levels was registered in the subpopulations of apoptotic cells. However the binase-induced RNA decrease did not correlate with apoptosis. Kit-transformed cells with binase-induced RNA decrease retained viability if the interleukin-dependent proliferation pathway was .