Đang chuẩn bị liên kết để tải về tài liệu:
Báo cáo khoa học: "124I-HuCC49deltaCH2 for TAG-72 antigen-directed positron emission tomography (PET) imaging of LS174T colon adenocarcinoma tumor implants in xenograft mice: preliminary results"
Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ
Tải xuống
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: 124I-HuCC49deltaCH2 for TAG-72 antigen-directed positron emission tomography (PET) imaging of LS174T colon adenocarcinoma tumor implants in xenograft mice: preliminary results | Zou et al. World Journal of Surgical Oncology 2010 8 65 http www.wjso.eom content 8 1 65 WORLD JOURNAL OF SURGICAL ONCOLOGY RESEARCH Open Access 124I-HuCC49deltaCH2 for TAG-72 antigen-directed positron emission tomography PET imaging of LS174T colon adenocarcinoma tumor implants in xenograft mice preliminary results Peng Zou1 2 Stephen P Povoski 1 3 Nathan C Hall4 Michelle M Carlton4 George H Hinkle4 5 Ronald X Xu6 Cathy M Mojzisik4 Morgan A Johnson4 Michael V Knopp4 Edward W Martin Jr3 Duxin Sun1 2 Abstract Background 18F-fluorodeoxyglucose positron emission tomography 18F-FDG-PET is widely used in diagnostic cancer imaging. However the use of 18F-FDG in PET-based imaging is limited by its specificity and sensitivity. In contrast anti-TAG tumor associated glycoprotein -72 monoclonal antibodies are highly specific for binding to a variety of adenocarcinomas including colorectal cancer. The aim of this preliminary study was to evaluate a complimentary determining region CDR -grafted humanized CH2-domain-deleted anti-TAG-72 monoclonal antibody HuCC49deltaCH2 radiolabeled with iodine-124 124I as an antigen-directed and cancer-specific targeting agent for PET-based imaging. Methods HuCC49deltaCH2 was radiolabeled with 124I. Subcutaneous tumor implants of LS174T colon adenocarcinoma cells which express TAG-72 antigen were grown on athymic Nu Nu nude mice as the xenograft model. Intravascular i.v. and intraperitoneal i.p. administration of 124I-HuCC49deltaCH2 was then evaluated in this xenograft mouse model at various time points from approximately 1 hour to 24 hours after injection using microPET imaging. This was compared to i.v. injection of 18F-FDG in the same xenograft mouse model using microPET imaging at 50 minutes after injection. Results At approximately 1 hour after i.v. injection 124I-HuCC49deltaCH2 was distributed within the systemic circulation while at approximately 1 hour after i.p. injection 124I-HuCC49deltaCH2 was distributed within the peritoneal .