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Báo cáo khoa học: "Micropropagation and ex vitro rooting of several clones of late-flushing Quercus robur "

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: Micropropagation and ex vitro rooting of several clones of late-flushing Quercus robur L. | Ann Sci For 1993 50 Suppl 1 319S-322S Elsevier INRA 319s Original article Micropropagation and ex vitro rooting of several clones of late-flushing Quercus robur L A Meier-Dinkel B Becker D Duckstein Niedersãchsische Forstliche Versuchsanstalt Abt Forstpflanzenziichtung 3513 Staufenberg-Escherode Germany Summary Green acorns from 11 selected late-flushing Quercus robur trees were used as initial explants for micropropagation. From 60 acorns 45 clones which produced shoots of suitable quality for ex vitro rooting were obtained. Half-sib clones derived form one mother tree produced an average of 409 microcuttings within 8 months. Half-sib clones of the other 10 trees produced only 11188 shoots per clone. Microcuttings were rooted ex vitro after treatment with rooting powder containing 0.5 indole-3-butyric acid or 1.0 indole acetic acid. Shoots derived form subcultured shoot tips and nodal segments had a low rooting and survival rate 21 after 4 months. 56 of shoots derived from subcultured basal segments with a callus rooted and survived. tissue culture micropropagation in vitro propagation I Quercusl ex vitro rooting Resume Micropropagation et enracinement ex vitro de plusieurs clones de Quercus robur L à débourrement tardif. Des glands encore verts ont été récoltés sur 11 clones de Quercus robur à débourrement tardif et utilises comme materiel de depart pour la micropropagation. Sur 60 glands 45 se sont avérés de qualité suffisante pour être enracinés ex vitro. Des clones demi-freres d un seul arbre mere ont produit 409 microboutures en 8 mois. Des clones demi-freres issus des 10 autres arbres n ont produit que de 11 à 188 pousses par clone. Les microboutures ont été enraci-nées ex vitro après avoir été enduites d une poudre comprenant 0 5 d acide indole butyrique et 1 0 d acide indole acétique. Les pousses issues des cultures ultérieures des parties apicales et des segments de tiges comprenant un noeud manifestaient un faible enracinement et taux de sur-vie 21 après