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báo cáo hóa học:" Resveratrol prevents inflammation-dependent hepatic melanoma metastasis by inhibiting the secretion and effects of interleukin-18"
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Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Resveratrol prevents inflammation-dependent hepatic melanoma metastasis by inhibiting the secretion and effects of interleukin-18 | Salado et al. Journal of Translational Medicine 2011 9 59 http www.translational-medicine.eom content 9 1 59 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Resveratrol prevents inflammation-dependent hepatic melanoma metastasis by inhibiting the secretion and effects of interleukin-18 Clarisa Salado1 Elvira Olaso2 Natalia Gallot3 Maria Valcarcel3 Eider Egilegor3 Lorea Mendoza3 and Fernando Vidal-Vanaclocha4 Abstract Background Implantation and growth of metastatic cancer cells at distant organs is promoted by inflammationdependent mechanisms. A hepatic melanoma metastasis model where a majority of metastases are generated via interleukin-18-dependent mechanisms was used to test whether anti-inflammatory properties of resveratrol can interfere with mechanisms of metastasis. Methods Two experimental treatment schedules were used 1 Mice received one daily oral dose of 1 mg kg resveratrol after cancer cell injection and the metastasis number and volume were determined on day 12. 2 Mice received one daily oral dose of 1 mg kg resveratrol along the 5 days prior to the injection of cancer cells and both interleukin-18 IL-18 concentration in the hepatic blood and microvascular retention of luciferase-transfected B16M cells were determined on the 18th hour. In vitro primary cultured hepatic sinusoidal endothelial cells were treated with B16M-conditioned medium to mimic their in vivo activation by tumor-derived factors and the effect of resveratrol on IL-18 secretion on vascular cell adhesion molecule-1 VCAM-1 expression and on tumor cell adhesion were studied. The effect of resveratrol on melanoma cell activation by IL-18 was also studied. Results Resveratrol remarkably inhibited hepatic retention and metastatic growth of melanoma cells by 50 and 75 respectively. The mechanism involved IL-18 blockade at three levels First resveratrol prevented IL-18 augmentation in the blood of melanoma cell-infiltrated livers. Second resveratrol inhibited IL-18-dependent expression