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Báo cáo hóa học: " Application of real-time PCR to quantify hepatitis B virus DNA in chronic carriers in The Gambia"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Application of real-time PCR to quantify hepatitis B virus DNA in chronic carriers in The Gambia | Virology Journal BioMed Central Research Open Access Application of real-time PCR to quantify hepatitis B virus DNA in chronic carriers in The Gambia Maimuna E Mendy 1 2 Steve Kaye1 3 Marianne van der Sande1 4 Pura Rayco-Solon1 5 Pauline A Waight1 6 Deborah Shipton1 Dorka Awi1 Paul Snell1 Hilton Whittle1 and Samuel J McConkey1 7 Address 1Medical Research Council Atlantic Boulevard Fajara P O Box 273 Banjul The Gambia 2Viral Disease programme Medical Research Council Atlantic Boulevard Fajara P O Box 273 Banjul The Gambia 3Imperial college London UK 4RIVM Bithoven The Netherlands 5Nutrition centre of the Philippines Philippines 6National Protection Agency Collindale London UK and 7Royal College of Surgeons in Ireland Dublin Ireland Email Maimuna E Mendy - mmendy@mrc.gm Steve Kaye - stevekayegambia@yahoo.com Marianne van der Sande - Marianne.van.der.Sande@rivm.nl Pura Rayco-Solon - Pura.Solon@lshtm.ac.uk Pauline A Waight - Pauline.Kaye@HPA.org.uk Deborah Shipton - shiptondeb@hotmail.com Dorka Awi - dorka_awi@yahoo.co.uk Paul Snell - psnell@mrc.gm Hilton Whittle - hwhitle@mrc.gm Samuel J McConkey - sammcconkey@rcsi.ie Corresponding author Published 04 April 2006 Received 01 November 2005 Accepted 04 April 2006 Virology Journal 2006 3 23 doi 10.1 186 1743-422X-3-23 This article is available from http www.virologyj.cOm content 3 1 23 2006 Mendy et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Aim The study aimed at developing a real-time quantitative PCR assay to monitor HBV serum virus load of chronic carriers enrolled in therapeutic trials. Method Quantitative real-time PCR assay was carried out using SYBR-Green signal detection and primers specific to the S gene. Thermal cycling was performed in an .