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Báo cáo hóa học: " Differential expression of papillomavirus L1 proteins encoded by authentic and codon modified L1 genes in methylcellulose-treated mouse keratinocytes"
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Tham khảo tài liệu 'báo cáo hóa học: " differential expression of papillomavirus l1 proteins encoded by authentic and codon modified l1 genes in methylcellulose-treated mouse keratinocytes"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Virology Journal BioMed Central Open Access Short report Differential expression of papillomavirus L1 proteins encoded by authentic and codon modified L1 genes in methylcellulose-treated mouse keratinocytes Xiao Wang Bo Li and Kong-Nan Zhao Address Diamantina Institute for Cancer Immunology Metabolic Medicine University of Queensland Research Extension Building 1 Princess Alexandra Hospital Woolloongabba Queensland 4102 Australia Email XiaoWang-xxxwang1@uq.edu.au Bo Li - uqbli3@uq.edu.au Kong-Nan Zhao - k.zhao@uq.edu.au Corresponding author Published 25 November 2007 Received 5 October 2007 Accepted 25 November 2007 Virology Journal 2007 4 127 doi 10.1186 1743-422X-4-127 This article is available from http www.virologyj.com content 4 1 127 2007 Wang et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Papillomaviruses PVs are double-stranded DNA viruses that infect keratinocytes in differentiating epithelia and induce hyperproliferative lesions. Here we used methylcellulose to induce cell differentiation of primary mouse keratinocytes KCs in in vitro culture and assessed the expression of authentic and codon-modified version of LI capsid genes from two PV types HPV6b and BPVI . Based on the quantitative RT-PCR analysis methylcellulose treatment did not influence the transcriptional expression of both authentic and codon-modified LI genes in KCs. Western blot showed that methylcellulose significantly increased the levels of the LI proteins expressed from two authentic LI genes. Conversely methylcellulose dramatically decreased LI protein expression in KCs transfected with two codon-modified LI expression constructs. These data suggest